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ID 55725
フルテキストURL
著者
Hayakawa, Tohru Graduate School of Natural Science and TechnologyOkayama University
Yoneda, Naoya Graduate School of Natural Science and TechnologyOkayama University
Okada, Kouji Graduate School of Natural Science and TechnologyOkayama University
Higaki, Ayuko Graduate School of Natural Science and TechnologyOkayama University
Mohammad Tofazzal Hossain Howlader Graduate School of Natural Science and TechnologyOkayama University
Ide, Toru Graduate School of Natural Science and TechnologyOkayama University
抄録
A 2,175-bp modified gene (cry11Ba-S1) encoding Cry11Ba from Bacillus thuringiensis subsp. jegathesan was designed and the recombinant protein was expressed as a fusion protein with glutathione S-transferase in Escherichia coli. The recombinant Cry11Ba was highly toxic against Culex pipiens mosquito larvae, being nine and 17 times more toxic than mosquitocidal Cry4Aa and Cry11Aa from Bacillus thuringiensis subsp. israelensis, respectively. Interestingly, a further increase in the toxicity of the recombinant Cry11Ba was achieved by mixing with Cry4Aa, but not with Cry11Aa. These findings suggested that Cry11Ba worked synergistically with Cry4Aa, but not with Cry11Aa, in exhibiting toxicity against C. pipiens larvae. On the other hand, the amount of Cry toxin bound to brush border membrane vesicles (BBMVs) did not significantly change between individual toxins and the toxin mixtures, suggesting that the increase in toxins binding to BBMVs was not a reason for the observed synergistic effect. It is generally accepted that synergism of toxins is a potentially powerful tool for enhancing insecticidal activity and managing Cry toxin resistance in mosquitoes. The mixture of Cry4Aa and Cry11Ba in order to increase toxicity would be very valuable in terms of mosquito control.
キーワード
Cry toxin
Synergistic toxicity
Insect pest control
Biological control
Disease vector
備考
This is an Accepted Manuscript of an article published by Springer Nature
発行日
2017-02
出版物タイトル
Applied Entomology and Zoology
52巻
1号
出版者
Springer Japan
開始ページ
61
終了ページ
68
ISSN
0003-6862
NCID
AA00543238
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
論文のバージョン
author
DOI
Web of Sience KeyUT
関連URL
isVersionOf https://doi.org/10.1007/s13355-016-0454-z