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ID 50260
フルテキストURL
著者
Inoue, Genki Department of Chemistry, Division of Earth, Life, and Molecular Sciences, Graduate School of Natural Science and Technology, Okayama University
Kaneta, Takashi Department of Chemistry, Division of Earth, Life, and Molecular Sciences, Graduate School of Natural Science and Technology, Okayama University
Takayanagi, Toshio Department of Life System, Institute of Technology and Science, The University of Tokushima
Kakehi, Junichi Department of Biological Science, Division of Earth, Life, and Molecular Sciences, Graduate School of Natural Science and Technology, Okayama University
Motose, Hiroyasu Department of Biological Science, Division of Earth, Life, and Molecular Sciences, Graduate School of Natural Science and Technology, Okayama University
Takahashi, Taku Department of Biological Science, Division of Earth, Life, and Molecular Sciences, Graduate School of Natural Science and Technology, Okayama University
抄録
Herein, we report a novel method for the determination of polyamines in a sample extracted from Arabidopsis thaliana by capillary electrophoresis (CE) using salicylaldehyde-5-sulfonate (SAS) as a derivatizing reagent. An aldehyde group of SAS forms a Schiff base with amino groups of aliphatic polyamines, resulting in an anionic species with an absorption band in the ultraviolet region. The derivatization method was straightforward since the derivatives were formed by mixing a sample with the derivatizing reagent at a neutral pH. In addition, the negative charges induced by SAS led to a high resolution with a short analysis time. This method permitted the separation of five polyamines, which play important roles in plants. However, further improvement in sensitivity was needed for the determination of the polyamines in plant samples. Therefore, the CE method was coupled with solid-phase extraction (SPE) using an ion-pairing formation with sodium dodecyl benzene sulfonate. The SPE method improved the concentration limits of detection to sub-μM levels, which corresponded with a 10-fold enhancement. The calibration curves for cadaverine, putrescine, and spermidine were linear with concentrations that ranged from 1 to 20 μM and correlation coefficients (R2) were greater than 0.998. The proposed method was applied to the determination of spermidine in a plant sample, Arabidopsis thaliana.
発行日
2013-05
出版物タイトル
Analytical Methods
5巻
11号
出版者
RSC Publishing
開始ページ
2854
終了ページ
2859
ISSN
1759-9660
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
著作権者
©The Royal Society of Chemistry 2013
論文のバージョン
author
査読
有り
DOI