ID | 11644 |
Eprint ID | 11644
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フルテキストURL | |
タイトル(別表記) | 肝外組織由来正常細胞はラット移植肝における肝細胞再生へわずかに寄与する
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著者 |
冨山 浩司
岡山大学
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抄録 | BACKGROUND: It is now well established that various adult somatic tissues harbor multipotent stem cells that can differentiate into a broad variety of cell types of all three germ layer origins. It remains controversial, however, whether they are a reservoir of cells utilized for emergent tissue repair or simply a vestige of evolution and, if the former is the case, to what extent they can potentially contribute to reconstitution of a specific organ. To get an insight in such a direction, we examined the extent of contribution of naive intact cells of extrahepatic origin to hepatocyte reconstitution in the transplanted liver with or without injury in the rat. METHODS: Liver from wild-type donor rats was transplanted to green fluorescent protein (GFP)-transgenic rats, and GFP-positive hepatocytes were examined with or without liver injury. RESULTS: The proportion of GFP-positive hepatocytes in the transplanted noninjured liver linearly increased by 0.0048% per week, that is, approximately 5 x 10(3) hepatocytes of extrahepatic origin were generated per day. Liver injury induced by treatment with 2-acetylaminofluorene and CCl4 or the additional application of hepatocyte growth factor did not further increase the percentage of GFP-positive hepatocytes. CONCLUSION: The present results indicate that cells derived from nonmanipulated extrahepatic tissues appreciably contribute, though limitedly, to hepatocyte reconstitution in the liver of the rat.
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発行日 | 2007-03-23
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出版物タイトル | |
資料タイプ |
学位論文
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学位授与番号 | 甲第3301号
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学位授与年月日 | 2007-03-23
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学位・専攻分野 |
博士(医学)
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授与大学 | 岡山大学
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オフィシャル URL | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17353784&dopt=Abstract
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言語 |
日本語
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論文のバージョン | none
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査読 |
不明
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