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ID 10613
Eprint ID
10613
フルテキストURL
Thumnail O004086.pdf 97.8 KB
タイトル(別表記)
透過型電子顕微鏡法のためのマイクロウェーブ照射による試料内鉄コロイドの作製法
著者
中谷 聡 岡山大学
抄録
We attempted to prepare colloidal iron within tissues by means of microwave irradiation. Mouse tissue blocks were fixed with a mixture of paraformaldehyde and ferric chloride in a cacodylate buffer, immersed in a cacodylate buffered ferric chloride solution, and irradiated in a microwave processor. Colloidal iron was prepared within tissues or cells, and was observed in the form of electron dense fine granules (1-2 nm in diameter) by transmission electron microscopy. Collagen fibrils in the connective tissue showed colloidal iron deposition at regular periodical intervals. Cells in the splenic tissue showed that fine colloidal granules were deposited on the ribosomes but not on the nuclear chromatin. This finding suggests that ferric ions could not diffuse into the nucleus, which was surrounded by the nuclear envelope. The podocyte processes of the renal glomerulus were stained diffusedly. Though this microwave in situ colloidal iron preparation method has some limitations, it is convenient for use in biomedical specimen preparation in transmission electron microscopy.
キーワード
colloidal iron
microwave
histochemistry
transmission electron microscopy
備考
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16508690&dopt=Abstract
発行日
2006-03-24
出版物タイトル
資料タイプ
学位論文
学位授与番号
乙第4086号
学位授与年月日
2006-03-24
学位・専攻分野
博士(医学)
授与大学
岡山大学
オフィシャル URL
http://escholarship.lib.okayama-u.ac.jp/cgi/viewcontent.cgi?article=1041&context=amo
言語
Japanese
論文のバージョン
none
査読
不明