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ID 4688
Eprint ID
4688
フルテキストURL
Thumnail K002093.pdf 173 KB
タイトル(別表記)
ラット海馬の錐体細胞におけるDNAトポイソメラーゼⅡβ発現の領域による違い
著者
前田 幸英 岡山大学
抄録
A detailed analysis of the differential expression of a nuclear enzyme, DNA topoisomerase (topo) IIβ, was performed in the rat hippocampal pyramidal layer. Three-dimensional (3-D) reconstruction from serial sections immunostained with anti-topo IIβ antibody showed that the immunoreactivity was apparently weak in the entire CA3 region. Almost all CA1 pyramidal cells showed similar immunoreactivity to that seen in the dentate granular cells, the subicular neurons, and the cerebral neocortical neurons. In addition, immunoblotting analysis in the adult dorsal hippocampus showed that the expression level of topo IIβ in the CA3 was significantly lower than that in the CA1 region. The dissociation in the expression level between CA1 and CA3 occurred in postnatal days 4 (P4) through P6. The present finding suggests that the enzyme is possibly involved in activities of the hippocampal pyramidal neurons.
キーワード
DNA topoisomerase IIβ
Hippocampal pyramidal cells
Immunohistochemistry
3-D reconstruction
Western blotting
Postnatal development
備考
掲載順位 77 http://dx.doi.org/10.1016/S0168-0102(00)00098-5
発行日
2000-03-31
出版物タイトル
資料タイプ
学位論文
学位授与番号
甲第2093号
学位授与年月日
2000-03-31
学位・専攻分野
博士(医学)
授与大学
岡山大学
オフィシャル URL
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10771107&dopt=Abstract
言語
Japanese
論文のバージョン
none
査読
不明