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ID 32848
JaLCDOI
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著者
Huang, Zhenyu College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
Liu, Qingping College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
Li, Wenzhe College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
Wang, Renjun College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
Wang, Dan College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
Zhang, Yingbiao College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
Zhang, Fan College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
Chi, Yan College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
Liu, Zhe College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
Matsuura, Eiji Department of Cell Chemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Liu, Zibo The No. 10 Senior High School of Dalian Economical and Technological Development Area
Zhang, Qiming College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
抄録

We investigated the molecular mechanisms responsible for the induction of apoptosis in mouse monocytic macrophage cell line J774A.1 stimulated by 7-ketocholesterol (7-KC). Cell apoptosis was detected by Annexin V-propidium iodide (PI) staining. The DNA-binding activity of nuclear factor kappa B (NF-kappaB) was assessed by electrophoretic mobility shift assay (EMSA). Results showed that 7-KC-stimulation in J774A.1 cells activated NF-kappaB, which is involved in cell apoptosis, in a time- and dose-dependent manners. 7-KC was also found to increase the binding activity of NF-kappaB to specific DNA binding sites, a possible mechanism for the induction of the cell apoptosis. Moreover, these effects were partially inhibited by pyrrolidine dithiocarbamate (PDTC), an NF-kappaB inhibitor. Taken together, 7-KC may be an important factor in atherosclerosis due to the ability of 7-KC to induce cell apoptosis, which is at least partially mediated through the activation of NF-kappaB.

キーワード
7-KC
NF-?B
apoptosis
atherosclerosis
Amo Type
Original Article
発行日
2010-04
出版物タイトル
Acta Medica Okayama
64巻
2号
出版者
Okayama University Medical School
開始ページ
85
終了ページ
93
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT