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ID 48565
JaLCDOI
フルテキストURL
著者
Zhang, Kai Department of Bacteriology, Okayama University Graduate School of Medicine
Yamamoto, Yumiko Department of Bacteriology, Okayama University Graduate School of Medicine
Suzuki, Tomonori Department of Bacteriology, Okayama University Graduate School of Medicine
Yokota, Kenji Graduate School of Health Sciences, Okayama University
Ma, Shaobo Department of Bacteriology, Okayama University Graduate School of Medicine
Nengah Dwi Fatmawati, Ni Department of Bacteriology, Okayama University Graduate School of Medicine
Oguma, Keiji Department of Bacteriology, Okayama University Graduate School of Medicine
抄録
Cultured Clostridium botulinum strains produce progenitor toxins designated as 12S, 16S, and 19S toxins. The 12S toxin consists of a neurotoxin (NTX, 7S) and a non-toxic non-hemagglutinin (NTNH). The 16S and 19S toxins are formed by conjugation of the 12S toxin with hemagglutinin (HA), and the 19S toxin is a dimer of the 16S toxin. Type A cultures produce all 3 of these progenitor toxins, while type E produces only the 12S toxin. The 7S toxin is cleaved into heavy (H) and light (L) chains by a protease(s) in some strains, and the H chain has 2 domains, the N-terminus (Hn) and C-terminus (Hc). It has been reported that type A toxins bind to the intestinal cells or cultured cells via either HA or Hc. In this study, we investigated the binding of type A and E toxins to Caco-2 cells using Western blot analysis. Both the type E 7S and 12S toxins bound to the cells, with the 7S toxin binding more strongly, whereas, in the type A strain, only the 16S/19S toxins showed obvious binding. Pre-incubation of the type E 7S toxin with IgG against recombinant type E Hc significantly inhibited the 7S toxin binding, indicating that Hc might be a main binding domain of the type E toxin.
キーワード
Clostridum botulinum
neurotoxins
Caco-2
binding
Hc
Amo Type
Original Article
発行日
2012-06
出版物タイトル
Acta Medica Okayama
66巻
3号
出版者
Okayama University Medical School
開始ページ
253
終了ページ
261
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
著作権者
CopyrightⒸ 2012 by Okayama University Medical School
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT
関連URL
http://ousar.lib.okayama-u.ac.jp/metadata/48451