フルテキストURL ijo_49_3_877.pdf
著者 Yamada, Hiroshi| Takeda, Tetsuya| Michiue, Hiroyuki| Abe, Tadashi| Takei, Kohji|
抄録 The endocytic protein dynamin participates in the formation of actin-based membrane protrusions such as podosomes, pseudopodia, and invadopodia, which facilitate cancer cell migration, invasion, and metastasis. However, the role of dynamin in the formation of actin-based membrane protrusions at the leading edge of cancer cells is unclear. In this study, we demonstrate that the ubiquitously expressed dynamin 2 isoform facilitates cell migration by stabilizing F-actin bundles in filopodia of the lung cancer cell line H1299. Pharmacological inhibition of dynamin 2 decreased cell migration and filopodial formation. Furthermore, dynamin 2 and cortactin mostly colocalized along F-actin bundles in filopodia of serum-stimulated H1299 cells by immunofluorescent and immunoelectron microscopy. Knockdown of dynamin 2 or cortactin inhibited the formation of filopodia in serum-stimulated H1299 cells, concomitant with a loss of F-actin bundles. Expression of wild-type cortactin rescued the punctate-like localization of dynamin 2 and filopodial formation. The incubation of dynamin 2 and cortactin with F-actin induced the formation of long and thick actin bundles, with these proteins colocalizing at F-actin bundles. A depolymerization assay revealed that dynamin 2 and cortactin increased the stability of F-actin bundles. These results indicate that dynamin 2 and cortactin participate in cell migration by stabilizing F-actin bundles in filopodia. Taken together, these findings suggest that dynamin might be a possible molecular target for anticancer therapy.
発行日 2016-06-30
出版物タイトル International Journal of Oncology
49巻
3号
出版者 Spandidos
開始ページ 877
終了ページ 886
ISSN 1019-6439
NCID AA10992511
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン publisher
PubMed ID 27572123
DOI 10.3892/ijo.2016.3592
Web of Sience KeyUT 000382447300003
関連URL isVersionOf https://doi.org/10.3892/ijo.2016.3592
著者 山田 浩司| Padilla-Parra, Sergi| 朴 宣奏| 伊藤 俊樹| Chaineau, Mathilde| Monaldi, Ilaria| Cremona, Ottavio| Benfenati, Fabio| Camilli, Pietro De| Coppey-Moisan, Maïté| Tramier, Marc| Galli, Thierry| 竹居 孝二|
発行日 2011-04-01
出版物タイトル 岡山医学会雑誌
123巻
1号
資料タイプ 学術雑誌論文
著者 Moriyama, Yoshinori| Hayashi, Mitsuko| Yamada, Hiroshi| Yatsushiro, Shouki| Ishio, Shougo| Yamamoto, Akitsugu|
発行日 2001-01
出版物タイトル The Journal of Experimental Biology
203巻
1号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/30954
フルテキストURL fulltext.pdf
著者 Nakanishi, Akira| Abe, Tadashi| Watanabe, Masami| Takei, Kohji| Yamada, Hiroshi|
抄録 <p>Testicular Sertoli cells highly express dynamin 2 and amphiphysin 1. Here we demonstrate that dynamin 2 is implicated in phosphatidylserine (PS)-dependent phagocytosis in Sertoli cells. Immunofluorescence and dual-live imaging revealed that dynamin 2 and amphiphysin 1 accumulate simultaneously at ruffles. These proteins are specifically bound <i>in vitro</i>. Over-expression of dominant negative dynamin 2 (K44A) inhibits liposome-uptake and leads to the mis-localization of amphiphysin 1. Thus, the cooperative function of dynamin 2 and amphiphysin 1 in PS-dependent phagocytosis is strongly suggested.</p>
キーワード dynamin amphiphysin phagocytosis testis
Amo Type Original Article
発行日 2008-12
出版物タイトル Acta Medica Okayama
62巻
6号
出版者 Okayama University Medical School
開始ページ 385
終了ページ 391
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
Web of Sience KeyUT 000262025000005