JaLCDOI 10.18926/AMO/30799
フルテキストURL fulltext.pdf
著者 Secilmis, Ata| Atci, Serare| Yaktubay, Naciye| Karatas, Yusuf| Gocmen, Cemil| Singirik, Ergin| Dikmen, Atilla| Baysal, Firuz|
抄録 <p>&#8195;The aim of the study was to investigate whether Na<sup>+</sup> channels play a role in the twitch component of the response of the isolated frog rectus abdominis to Ca<sup>2+</sup>-free Ringer solution with 0.2 mM Na<sub>2</sub>EDTA by using tetrodotoxin and some other well known drugs that exhibit a blocking action on Na<sup>+</sup> channels. In the presence of 5 x 10<sup>-7</sup> M tetrodotoxin, the twitch component, measured isotonically, disappeared. Although 10<sup>-7</sup> M d-tubocurarine was found to be ineffective, a complete blockage of twitch amplitude was observed at 5 x 10<sup>-6</sup> M concentration of the drug. The inhibitory action of d-tubocurarine on twitch response was not antagonized by 10<sup>-6</sup> and 10<sup>-5</sup> M carbachol. Propranolol (10<sup>-6</sup> - 10<sup>-5</sup> M), lidocaine (2 x 10<sup>-6</sup> - 10<sup>-5</sup> M), quinine (10<sup>-6</sup> - 2 x 10<sup>-5</sup> M) and quinidine (10<sup>-6</sup> - 2 x 10<sup>-5</sup> M) inhibited maximal twitch amplitude in a concentration dependent manner. These findings strongly suggest that activation of tetrodotoxin sensitive Na<sup>+</sup> channel may play a primary role at twitch generation during exposure of the frog rectus abdominis to Ca<sup>2+</sup>-free Ringer solution with Na<sub>2</sub> EDTA.</p>
キーワード tetrodotoxin Ca<sup>2+</sup>-free medium with Na<sub>2</sub> EDTA isolated frog rectus abdominis Na<sup>+</sup> channel blocking activity
Amo Type Article
発行日 1997-06
出版物タイトル Acta Medica Okayama
出版者 Okayama University Medical School
開始ページ 115
終了ページ 120
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 9227789
Web of Sience KeyUT A1997XJ12700001