JaLCDOI 10.18926/AMO/32310
フルテキストURL fulltext.pdf
著者 Emdadul, Haque M| Fujisawa, Tomomi| Yamamoto, Masamichi| Ohmori, Shinji|
抄録 <p>We present here a reliable and sensitive method for the determination of acidic opines such as meso-alanopine, beta-alanopine, tauropine and strombine in biological samples. Interfering primary amino acids were eliminated by reaction with o-phthalaldehyde, and the derivatized compounds were passed through Sep-Pak Plus PS-1 cartridges. The acidic opines were recovered by flushing the cartridges with water, then determined by high performance liquid chromatography after a second derivatization with phenylisothiocyanate. All 4 acidic opines were detected within 30 min. This method ensured good separation and guaranteed almost full recovery of all acidic opines. This method was applied to analyze opines in marine animals and to test whether opines are metabolized in the livers of the rat and fish.</p>
キーワード opines high-performance liquid chromatography determination marine animals phenylisothiocyanate Sep-Pak cartridge
Amo Type Article
発行日 2000-02
出版物タイトル Acta Medica Okayama
54巻
1号
出版者 Okayama University Medical School
開始ページ 1
終了ページ 8
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 10709616
Web of Sience KeyUT 000085526000001