著者 Ichiyama, Kenji| Mitsuzumi, Hitoshi| Zhong, Ming| Tai, Akihiro| Tsuchioka, Akihiro| Kawai, Saeko| Yamamoto, Itaru| Gohda, Eiichi|
発行日 2009-02-21
出版物タイトル Immunology Letters
122巻
2号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/32828
フルテキストURL fulltext.pdf
著者 Liu, Jie| Yagi, Takahito| Sadamori, Hiroshi| Matsukawa, Hiroyoshi| Sun, Dong-Sheng| Mitsuoka, Naoshi| Yamamura, Masao| Matsuoka, Junji| Jin, Zaishun| Yamamoto, Itaru| Tanaka, Noriaki|
抄録 <p>Controversy exists over whether the predominant cell death of hepatocytes is due to apoptosis or necrosis after ischemia/reperfusion injury. In this study we investigated the predominant cell death of hepatocytes after cold ischemia/reperfusion injury using the Annexin V-based assay, and evaluated the anti-apoptotic effect of ascorbic acid 2-glucoside (AA-2G) added to the University of Wisconsin solution (UW solution) in rat liver transplantation. The retrieved liver was preserved in 4 UW solution for 24 h, and then transplanted orthotopically to the syngeneic Wistar recipient. The animals were divided into 2 groups, a control group (n=10), in which liver grafts were preserved in UW solution (4), and an AA-2G group (n=10), in which liver grafts were preserved in UW solution (4) with AA-2G (100 ug/ml). The serum AST level 4 h after reperfusion in the control group was significantly suppressed in the AA-2G group, and the bile production of the liver graft in the AA-2G group was well recovered. The mean survival time in the AA-2G group was significantly improved compared with that in the control group. Annexin-V and Propidium iodide staining 4 h after reperfusion showed a significantly higher percentage of viable hepatocytes in the AA-2G group compared with the control group (93.4 +/- 2.0 vs. 80.3 +- 2.1%, P&#60;0.05). In the control group, the main cell death of hepatocytes was apoptosis (early apoptosis: 10.0 +- 4.7%, late apoptosis: 6.4 +/- 1.7%). The addition of AA-2G to the UW solution significantly inhibited both early and late apoptotic cell death 4 h after reperfusion (early apoptosis: 0.98 +/- 0.88%, late apoptosis: 2.2 +/- 1.1%). The expression of caspase 9 in the immunostaining of the liver graft was suppressed in the AA-2G group compared with in the control group. Our study using the Annexin V-based assay provided evidence that the predominant cell death of hepatocytes was apoptosis after 24 h cold ischemia/reperfusion injury in rat liver transplantation. The addition of AA-2G to the UW solution attenuated 24 h cold ischemia/reperfusion injury by inhibiting the apoptosis of hepatocytes.</p>
キーワード apoptosis ischemia/ reperfusion injury liver transplantation ascorbic acid 2- glucoside(AA-2G)
Amo Type Article
発行日 2003-10
出版物タイトル Acta Medica Okayama
57巻
5号
出版者 Okayama University Medical School
開始ページ 209
終了ページ 216
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 14679398
Web of Sience KeyUT 000186186000001