start-ver=1.4
cd-journal=joma
no-vol=64
cd-vols=
no-issue=2
article-no=
start-page=85
end-page=93
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=201004
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=7-Ketocholesterol Induces Cell Apoptosis by Activation of Nuclear Factor kappa B in Mouse Macrophages
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>We investigated the molecular mechanisms responsible for the induction of apoptosis in mouse monocytic macrophage cell line J774A.1 stimulated by 7-ketocholesterol (7-KC). Cell apoptosis was detected by Annexin V-propidium iodide (PI) staining. The DNA-binding activity of nuclear factor kappa B (NF-kappaB) was assessed by electrophoretic mobility shift assay (EMSA). Results showed that 7-KC-stimulation in J774A.1 cells activated NF-kappaB, which is involved in cell apoptosis, in a time- and dose-dependent manners. 7-KC was also found to increase the binding activity of NF-kappaB to specific DNA binding sites, a possible mechanism for the induction of the cell apoptosis. Moreover, these effects were partially inhibited by pyrrolidine dithiocarbamate (PDTC), an NF-kappaB inhibitor. Taken together, 7-KC may be an important factor in atherosclerosis due to the ability of 7-KC to induce cell apoptosis, which is at least partially mediated through the activation of NF-kappaB.</p>
en-copyright=
kn-copyright=

en-aut-name=HuangZhenyu
en-aut-sei=Huang
en-aut-mei=Zhenyu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=LiuQingping
en-aut-sei=Liu
en-aut-mei=Qingping
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=LiWenzhe
en-aut-sei=Li
en-aut-mei=Wenzhe
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=WangRenjun
en-aut-sei=Wang
en-aut-mei=Renjun
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=WangDan
en-aut-sei=Wang
en-aut-mei=Dan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=ZhangYingbiao
en-aut-sei=Zhang
en-aut-mei=Yingbiao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=ZhangFan
en-aut-sei=Zhang
en-aut-mei=Fan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=ChiYan
en-aut-sei=Chi
en-aut-mei=Yan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=LiuZhe
en-aut-sei=Liu
en-aut-mei=Zhe
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=MatsuuraEiji
en-aut-sei=Matsuura
en-aut-mei=Eiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=LiuZibo
en-aut-sei=Liu
en-aut-mei=Zibo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=ZhangQiming
en-aut-sei=Zhang
en-aut-mei=Qiming
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

affil-num=1
en-affil=
kn-affil=College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University

affil-num=2
en-affil=
kn-affil=College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University

affil-num=3
en-affil=
kn-affil=College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University

affil-num=4
en-affil=
kn-affil=College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University

affil-num=5
en-affil=
kn-affil=College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University

affil-num=6
en-affil=
kn-affil=College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University

affil-num=7
en-affil=
kn-affil=College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University

affil-num=8
en-affil=
kn-affil=College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University

affil-num=9
en-affil=
kn-affil=College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University

affil-num=10
en-affil=
kn-affil=Department of Cell Chemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

affil-num=11
en-affil=
kn-affil=The No. 10 Senior High School of Dalian Economical and Technological Development Area

affil-num=12
en-affil=
kn-affil=College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
en-keyword=7-KC
kn-keyword=7-KC
en-keyword=NF-?B
kn-keyword=NF-?B
en-keyword=apoptosis
kn-keyword=apoptosis
en-keyword=atherosclerosis
kn-keyword=atherosclerosis
END