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ID 52903
フルテキストURL
著者
Ichiyanagi, Tomoko Department of Immunology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Ichiyanagi, Kenji Division of Epigenomics and Development, Medical Institute of Bioregulation, Kyushu University
Ogawa, Ayako Division of Epigenomics and Development, Medical Institute of Bioregulation, Kyushu University
Kuramochi-Miyagawa, Satomi Department of Pathology, Medical School and Graduate School of Frontier Biosciences, Osaka University
Nakano, Toru Department of Pathology, Medical School and Graduate School of Frontier Biosciences, Osaka University
Chuma, Shinichiro Department of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto University
Sasaki, Hiroyuki Division of Epigenomics and Development, Medical Institute of Bioregulation, Kyushu University
Udono, Heiichiro Department of Immunology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences 科研費研究者番号
抄録
HSP90, found in all kingdoms of life, is a major chaperone protein regulating many client proteins. We demonstrated that HSP90α, one of two paralogs duplicated in vertebrates, plays an important role in the biogenesis of fetal PIWI-interacting RNAs (piRNA), which act against the transposon activities, in mouse male germ cells. The knockout mutation of Hsp90α resulted in a large reduction in the expression of primary and secondary piRNAs and mislocalization of MIWI2, a PIWI homolog. Whereas the mutation in Fkbp6 encoding a co-chaperone reduced piRNAs of 28–32 nucleotides in length, the Hsp90α mutation reduced piRNAs of 24–32 nucleotides, suggesting the presence of both FKBP6-dependent and -independent actions of HSP90α. Although DNA methylation and mRNA levels of L1 retrotransposon were largely unchanged in the Hsp90α mutant testes, the L1-encoded protein was increased, suggesting the presence of post-transcriptional regulation. This study revealed the specialized function of the HSP90α isofom in the piRNA biogenesis and repression of retrotransposons during the development of male germ cells in mammals.
発行日
2014-09-27
出版物タイトル
Nucleic Acids Research
42巻
19号
出版者
Oxford University Press
開始ページ
11903
終了ページ
11911
ISSN
0305-1048
資料タイプ
学術雑誌論文
言語
English
著作権者
© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
論文のバージョン
publisher
査読
有り
DOI