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ID 31682
JaLCDOI
フルテキストURL
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著者
Shinji, Toshiyuki Okayama University
Ujike, Kozo Okayama University
Ochi, Koji Okayama University
Kusano, Nobuchika Okayama University
Kikui, Tetsuya Okayama University
Matsumura, Naoki Okayama University
Emori, Yasuyuki Okayama University
Seno, Toshinobu Okayama University
Koide, Norio Okayama University
抄録
In studies of the pathogenesis of pancreatic fibrosis, pancreatic stellate cells (PSCs) have recently gained attention. In the present study, we established a new collagenase perfusion method through thoracic aorta cannulation to isolate PSCs, and we studied gene expression of TGF-beta1, type I collagen, and connective tissue growth factor using primary cultured PSCs. Our method facilitated PSC isolation, and by our new method, 4.3 +/- 1.2 x 10(6) PSCs were obtained from a rat. In comparing the expression of these genes with that of hepatic stellate cells (HSCs), we observed a similar pattern, although PSCs expressed type I collagen gene earlier than did HSCs. These results suggest that PSCs may play an important role in fibrosis of the pancreas, as HSCs do in liver fibrosis; in addition, PSCs may exist in a preactivated state or may be more easily activated than are HSCs. We also isolated the PSCs from a WBN/Kob rat, the spontaneous pancreatitis rat, and compared the gene expression with that from a normal rat.
キーワード
pancreatic stellate cell
transforming growth factor beta
connective tissue growth factor
collagenase perfusion
WBN/Kob rat
Amo Type
Article
発行日
2002-08
出版物タイトル
Acta Medica Okayama
56巻
4号
出版者
Okayama University Medical School
開始ページ
211
終了ページ
218
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
著作権者
Copyright© 2002 by Okayama University Medical School
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT