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ID 32674
JaLCDOI
フルテキストURL
著者
Kawaguchi, Mitsuhiko Okayama University
Koide, Norio Okayama University
Sakaguchi, Kohsaku Okayama University
Shinji, Toshiyuki Okayama University
Tsuji, Takao Okayama University
抄録
We showed that the combination of epidermal growth factor (EGF) and insulin is an essential supplement to Williams' #E medium for the formation of floating multicellular spheroids in primary culture of rat hepatocytes. Isolated hepatocytes assembled to form floating multicellular spheroids within 96 h through transient assembly of monolayer islands within the initial 24 h in dishes coated with liver-derived proteoglycans. However, the assembly of multicellular spheroids was severely suppressed in the absence of either EGF or insulin. The reduction of spheroid assembly was correlated with decreased attachment and subsequent decreased formation of monolayer islands within 24 h. The minimum amounts of EGF and insulin required for the formation of floating spheroids were 1 ng/ml and 0.4 microgram/ml, respectively. These results suggest that the enhancement of hepatocyte attachment provided by the combination of EGF and insulin during the early phase of culture is required for the formation of floating spheroids.
キーワード
multicellular spheroid
epidermal growth factor
insulin
rat hepatocyte
primary culture
Amo Type
Article
発行日
1992-06
出版物タイトル
Acta Medica Okayama
46巻
3号
出版者
Okayama University Medical School
開始ページ
195
終了ページ
201
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT