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ID 32053
JaLCDOI
フルテキストURL
著者
Hashimoto, Kozo Okayama University
Yunoki, Sho Okayama University
Hosogi, Hidemi Okayama University
Takahara, Jiro Okayama University
Ofuji, Tadashi Okayama University
抄録

Biologically active peptides and neurotransmitter substances were added to anterior pituitary cell cultures to examine the presence of corticotropin releasing factor (CRF)-like activity. Hypothalamic extract (HE) induced significant dose-related increase of ACTH, and the lowest effective dose was 0.01 HE/ml. Other tested substances including luteinizing hormone-releasing hormone, thyrotropin releasing hormone, melanocyte stimulating hormone release inhibiting factor, somatostatin, substance P, neurotensin, beta-endorphin. leu-enkephalin, met-enkephalin, bradykinin, norepinephrine, dopamine, serotonin, acetylcholine, histamine, gamma-amino butyric acid or gamma-hydroxy butyric acid showed no CRF-like activity. Relatively high doses of lysine vasopressin, arginine vasopressin and angiotensin II increased the release of ACTH in pituitary cell cultures, but the maximal ACTH response was markedly less than with HE. These results indicate that cultured anterior pituitary cells are sensitive and fairly specific in detecting CRF(s) comparing with other detecting procedures.

キーワード
pituitary cell cultures
corticotropin releasing factor
ACTH
neuropeptides
neurotransmitter substances
Amo Type
Article
発行日
1979-04
出版物タイトル
Acta Medica Okayama
33巻
2号
出版者
Okayama University Medical School
開始ページ
81
終了ページ
90
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
NAID