JaLCDOI 10.18926/AMO/30558
フルテキストURL fulltext.pdf
著者 Yamada, Gotaro| Takahashi, Takashi| Mizuno, Motowo| Sakamoto, Yuji| Kaga, Kazumi| Kobayashi, Toshinari| Nagashima, Hideo|
抄録 <p>A recently modified method using peroxidase labeled antibodies for light and electron microscopic demonstration of hepatitis B virus (HBV) was applied to the evaluation of hepatitis B surface antigen (HBsAg) on the surface of liver cells in biopsy specimens from 24HBsAg chronic carriers. Membranous distribution of HBsAg was demonstrated in diffuse or scattered hepatocytes in all 4 asymptomatic carriers and in 3 of the 20 patients with HBsAg-positive chronic active hepatitis or liver cirrhosis. In these patients with membranous expression of HBsAg, hepatitis B e antigen, Dane particles and DNA polymerase were often detected in sera, and large amounts of hepatitis B core antigen appeared in the liver. These results suggest that membrane-bound HBsAg may be expressed by the HBV genome. The ultrastructural study of liver cells showing membranous expression disclosed dense deposits of reaction product indicative of HBsAg on the cell membrane and/or on assembled particles within the extracellular space. In some hepatocytes showing both diffuse cytoplasmic and membranous expression of HBsAg, HBsAg-positive membrane of cisternae open to the intercellular space was connected with the liver cell membrane. These findings supported the conjecture that HBV associated antigens are integrated into the liver cell membrane.</p>
キーワード type B hepatitis membrane-bound HBsAg immunoelectron microscopy peroxidase-labeled antibody method.
Amo Type Article
発行日 1980-06
出版物タイトル Acta Medica Okayama
34巻
3号
出版者 Okayama University Medical School
開始ページ 175
終了ページ 187
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6447986
Web of Science KeyUT A1980KE59200005
JaLCDOI 10.18926/AMO/30557
フルテキストURL fulltext.pdf
著者 Ymadada, Gotaro| Sakamoto, Yuji| Mizuno, Motowo| Kobayashi, Toshinari| Nagashima, Hideo|
抄録 <p>In electron microscopic observation of a liver biopsy obtained from a hepatitis B surface antigen-positive patient, noncoated core particles were occasionally seen budding into the hepatocytic cisterni and many Dane particles were found in the pericanalicular vesicles of hepatocytes. Noncoated core particles were also localized in clusters within the bleb of microvilli. There were some core particles being protruded from microvilli into the lumen of bile canaliculi by budding. These findings suggest hepatitis B virus being released from the hepatocyte to the bile canaliculi by two different modes; through vesicle by reversed phagocytosis and from the microvilli by budding.</p>
キーワード type B hepatitis hepatitis B virus Dane particle bile canaliculi.
Amo Type Brief Note
発行日 1980-06
出版物タイトル Acta Medica Okayama
34巻
3号
出版者 Okayama University Medical School
開始ページ 203
終了ページ 207
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6447989
Web of Science KeyUT A1980KE59200008
JaLCDOI 10.18926/AMO/30540
フルテキストURL fulltext.pdf
著者 Yamada, Gotaro| Sakamoto, Yuji| Mizuno, Motowo| Kobayashi, Toshinari| Nagashima, Hideo|
抄録 The liver biopsy specimen from a patient with hepatitis B surface antigen was pbserved by electron microscopy. Dane particles, uncoated core particles and tubular forms were demonstrated in hepatocytes. Dane particles and tubular forms, approximately 25nm in diameter, were also found in the bile canaliculi. These findings suggest that hepatitis B virus and associated particles are released from hepatocytes into the bile duct.
キーワード hepatitis B virus bile canaliculus.
Amo Type Brief Note
発行日 1980-04
出版物タイトル Acta Medica Okayama
34巻
2号
出版者 Okayama University Medical School
開始ページ 143
終了ページ 146
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6450517
Web of Science KeyUT A1980JY15600010
JaLCDOI 10.18926/AMO/30331
フルテキストURL fulltext.pdf
著者 Yamada, Gotaro| Mizuno, Motowo| Kinoyama, Shingo| Nishihara, Takashi| Okushin, Hiroaki| Hyodo, Ichinosuke| Sakamoto, Yuji| Nagashima, Hideo|
抄録 <p>In 144 cases of hepatocellular carcinoma (HCC), 166 cases of cirrhosis without HCC and 142 cases of chronic hepatitis, we examined HBsAg, anti-HBs and anti-HBc in sera and compared the following factors between hepatitis B virus marker-negative and -positive patients: age, sex, alcohol consumption, family clustering of liver diseases, and histories of blood transfusion and post-transfusion hepatitis. Results of this study demonstrated several distinct differences in clinical backgrounds between non-B (negative for HBsAg, anti-HBs and anti-HBc) and B (positive for HBsAg) patients with HCC. Non-B patients were significantly older, had a lower frequency of familial tendencies for liver diseases, and more frequently had cancers other than HCC in their families. Some of these differences were also observed between non-B and B patients with cirrhosis and chronic hepatitis. Among patients with chronic hepatitis, the non-B patients had received blood transfusion or had post-transfusion hepatitis more frequently than the B patients. However, this difference was not apparent in patients with liver cirrhosis or HCC, suggesting that progression of non-A, non-B post-transfusion hepatitis to cirrhosis and HCC may not be as frequent as progression to chronic hepatitis.</p>
キーワード non-B hepatocellular carcinoma type non-A non-B hepatitis type B hepatitis post-transfusion hepatitis
Amo Type Article
発行日 1984-04
出版物タイトル Acta Medica Okayama
38巻
2号
出版者 Okayama University Medical School
開始ページ 181
終了ページ 191
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6328894
Web of Sience KeyUT A1984SN81800010
JaLCDOI 10.18926/AMO/30310
フルテキストURL fulltext.pdf
著者 Hyodo, Ichinosuke| Yamada, Gotaro| Nishihara, Takashi| Okushin, Hiroaki| Kinoyama, Shingo| Sakamoto, Yuji| Tobe, Kazuo| Nagashima, Hideo|
抄録 <p>The incidence of hepatitis A (HA), hepatitis B (HB), and non-A, non-B hepatitis (NANBH) was 27%, 30% and 43% among 73 patients with sporadic hepatitis. Epidemiological data (geographical distribution, seasonal variation, age, sex, and occupation) were not distinguishing of the type of hepatitis. Neither intrafamilial infection nor previous contact with viral hepatitis patients could be demonstrated in the NANBH cases. Fever and jaundice were less frequent in NANBH than in HA. Maximum levels of SGPT, serum bilirubin, ZTT, and gamma-globulin were significantly lower in NANBH than in HA and HB. Ten of 29 NANBH patients (35%) presented abnormal SGPT activities for more than 6 months, and four (14%) more than 12 months. In the ten patients with prolonged courses, jaundice was more frequent and maximum levels of SGPT were higher than in patients with transient courses. Histopathologic findings were not markedly different from those of HA and HB. Bile duct damage, fatty deposition, and giant multi-nucleated cells were recognized in 6, 12, and 2 NANBH patients, respectively. There were no characteristic ultrastructural changes in NANBH.</p>
キーワード acute hepatitis sporadic non-A non-B hepatitis liver histopathology liver ultrastructure
Amo Type Article
発行日 1984-08
出版物タイトル Acta Medica Okayama
38巻
4号
出版者 Okayama University Medical School
開始ページ 389
終了ページ 401
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6437147
Web of Science KeyUT A1984TG25900009