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ID 32813
JaLCDOI
フルテキストURL
著者
Sawayama, Tomoyuki Okayama University
Sakaguchi, Kohsaku Okayama University
Senoh, Tomonori Okayama University
Ohta, Takeyuki Okayama University
Nishimura, Mamoru Okayama University
Takaki, Akinobu Okayama University Kaken ID
Tsuji, Takao Okayama University
Shiratori, Yasushi Okayama University
抄録

In patients with hepatocellular carcinoma (HCC), natural killer (NK) cell activity decreases significantly, and the reduced activity may be associated with the progression of HCC. In this study we evaluated the effects of pulsing with interleukin (IL)-2 and/or IL-12 on the activation of freshly isolated peripheral blood lymphocytes (PBL) derived from patients with HCC. PBL obtained from 9 HCC patients, 4 liver cirrhosis patients, and 9 normal subjects were cultured in the presence of IL-2 and/or IL-12. After 24 h of incubation, the levels of interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha presented in the supernatants were determined by enzyme-linked immunosorbent assay (ELISA). The IFN-gamma and TNF-alpha production of PBL pulsed by a combination of IL-2 and IL-12 was significantly higher than those of PBL stimulated by either IL-2 or IL-12 alone. The mRNA encoding perforin, granzyme B, as well as IFN-gamma and TNF-alpha, were markedly enhanced in PBL stimulated with a combination of IL-12 and IL-2. The pulsing procedure of IL-12 in combination with IL-2 resulted in the increase of IFN-gamma and TNF-alpha, and the expression of perforin and granzyme B mRNA in PBL obtained from HCC patients, as well as in those obtained from normal subjects. These results indicate that adoptive immunotherapy based on PBL pulsed with a combination of IL-2 and IL-12 may be a promising adjunctive strategy for HCC treatment.

キーワード
hepatocellular carcinoma(HCC)
interleukin(IL)-2
interleukin(IL)-12
interferon(IFN)-r
granzyme B
Amo Type
Article
発行日
2003-12
出版物タイトル
Acta Medica Okayama
57巻
6号
出版者
Okayama University Medical School
開始ページ
285
終了ページ
292
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT