JaLCDOI 10.18926/AMO/31928
フルテキストURL fulltext.pdf
著者 Sakagami, Kenichi| Miyazaki, Masashi| Matsuoka, Junji| Shiozaki, Shigehiro| Saito, Shinya| Orita, Kunzo|
抄録 <p>An artificial liver support system for plasma exchange and plasma perfusion through BR-601 resin using a membrane separator was applied to 5 patients with postoperative liver failure. Percent absorption of total and direct bilirubin, and of bile acids were 77.1 +/- 6.4, 78.4 +/- 6.1, and 93.4 +/- 3.6%, respectively, when 250 ml of plasma was treated. Percent reductions in total and direct bilirubin, and in bile acids were 24.5 +/- 5.8, 25.5 +/- 5.8 and 30.9 +/- 8.5%, respectively. In contrast, percent reductions in total and direct bilirubin, and in bile acids by plasma exchange were 30.9 +/- 13.3, 34.5 +/- 12.5 and 24.2 +/- 8.5%, respectively. The coma grade was improved in 4 out of 5 cases, but unfortunately the patients did not recover. In conclusion, plasma perfusion through BR-601 resin is expected to play a promising role in artificial liver support systems because of its capacity to absorb bilirubin and bile acids.</p>
キーワード anion exchange resin (BR-601) postoperative liver failure artificial liver support
Amo Type Article
発行日 1986-10
出版物タイトル Acta Medica Okayama
40巻
5号
出版者 Okayama University Medical School
開始ページ 249
終了ページ 255
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 3788665
Web of Science KeyUT A1986E557800003
JaLCDOI 10.18926/AMO/31571
フルテキストURL fulltext.pdf
著者 Tanemoto, Kazuo| Sakagami, Kenichi| Orita, Kunzo|
抄録 <p>A newly introduced compound, EPC-K1, represents a phosphate diester linkage of vitamin E and vitamin C. The effect of EPC-K1 on the reperfusion injury was evaluated in a heterotopic cardiac transplantation model using syngenic combination rats. Prior to the warm ischemia, 12mg EPC-K1/kg was administered intravenously to donor rats. After 15 min of warm ischemic time, hearts were harvested and perfused with 4 degrees C saline. After completion of the transplantation, recipient rats were also treated with intravenous 12 mg EPC-K1/kg, before reperfusion. Saline was used instead of EPC-K1 for both donors and recipients in the control group. On the 7th post-transplantation day, graft survival was 7 out of 8 in EPC-K1 group, versus 1 out of 9 in the control group (p &#60; 0.001). Thiobarbituric acid-reactive substance levels in the recipient serum, three hours after reperfusion, were significantly limited, in the group in which EPC-K1 was administered only to donors. But it was not possible to clarify whether the effect of EPC-K1 is primarily at the donor or recipient levels at this time. These results indicate that EPC-K1 may reduce reperfusion injury after cardiac transplantation. This beneficial effect may be mediated by the hydroxyl radical scavenging properties of EPC-K1.</p>
キーワード EPC-K cardiac transplantation free redical scavenger reperfusion injury thiobarbituric acidreactive substances
Amo Type Article
発行日 1993-04
出版物タイトル Acta Medica Okayama
47巻
2号
出版者 Okayama University Medical School
開始ページ 121
終了ページ 127
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8506749
Web of Sience KeyUT A1993LA45200009
JaLCDOI 10.18926/AMO/31939
フルテキストURL fulltext.pdf
著者 Sakagami, Kenichi| Takeuchi, Hitoshi| Tsuboi, Katsutoshi| Matsumoto, Takamasa| Tanaka, Kohtaro| Ohsaki, Toshihide| Horimi, Tadashi| Fuchimoto, Sadanori| Orita, Kunzo|
抄録 <p>The survival rate of 19 patients who underwent living-related kidney transplantation after donor-specific blood transfusions (DST) was compared with that of 32 historical controls receiving transplants without DST. The graft survival rate of the DST group was 82% after two and three years. The graft survival rate of the DST group was significantly better than the 53% rate after two years obtained with the 32 historical controls (p less than 0.05). We tested sera from 16 DST-treated recipients to study the beneficial effect of DST on kidney allograft survival using the mixed lymphocyte culture (MLC) serum inhibition test. The results demonstrated that MLC inhibitory factors were induced in the serum of the recipient after completion of DST. This inhibition of MLC was observed by treatment of responder lymphocytes with serum obtained three weeks after DST plus rabbit complement. The inhibitory effect was also specific for responder cells in anti-donor MLC. Regarding the correlation with rejection episodes, these MLC inhibitory factors were often observed in the non-rejection group (p less than 0.05). The data suggest that such factors may be anti-idiotypic antibodies and be associated with prolonged graft survival.</p>
キーワード kidney transplantation donor-specific blood transfusion (DST) MLC inhibitory factors anti-idiotypic antibody.
Amo Type Article
発行日 1986-02
出版物タイトル Acta Medica Okayama
40巻
1号
出版者 Okayama University Medical School
開始ページ 39
終了ページ 43
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 2938423
Web of Science KeyUT A1986A190200006
著者 Sakagami, Kenichi|
発行日 1976-06
出版物タイトル Acta Medica Okayama
30巻
3号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/30864
フルテキストURL fulltext.pdf
著者 Uchida, Susumu| Sakagami, Kenichi| Orita, Kunzo|
抄録 <p>We investigated the effects of fractionated sera obtained from cancer patients by double filtration plasmapheresis (DFPP) plus antitumor agents on murine pulmonary metastasis. Fractions of the sera, in combination with natural human tumor necrosis factors (nTNF) and cyclophosphamide (Cy), were systemically administered to Lewis lung carcinoma-bearing mice. When the second filtrate (a plasma fraction containing substances composed of smaller molecular weight compounds) combined with low-dose nTNF (1,000 U/kg) and Cy (250 micrograms/kg) was administered to the mice, the degree of metastasis was significantly suppressed compared with the control group (p less than 0.01). In contrast, the discarded fluid (a plasma fraction containing larger molecular weight compounds) combined with the same doses of nTNF and Cy caused little inhibition of metastasis. Also, the discarded fluid significantly suppressed natural killer activity compared with normal sera (p less than 0.01). The results suggested that DFPP combined with nTNF and Cy is an efficient procedure to remove immunosuppressive factors from the sera of cancer-bearing hosts, to enhance the host antitumor immunity, and to suppress tumor proliferation.</p>
キーワード double filtration plasmapheresis serum fractions tumor necrosis factors cyclophosphamide synergistic effect
Amo Type Article
発行日 1989-12
出版物タイトル Acta Medica Okayama
43巻
6号
出版者 Okayama University Medical School
開始ページ 337
終了ページ 344
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 2624141
Web of Science KeyUT A1989CG27400004
JaLCDOI 10.18926/AMO/31041
フルテキストURL fulltext.pdf
著者 Tanaka, Kotaro| Sakagami, Kenichi| Orita, Kunzo|
抄録 <p>It is now well recognized that pre-transplant donor-specific blood transfusion (DST) has a beneficial effect on the survival of allografts. To determine the optimal interval between DST and transplantation, and to analyze the mechanisms of this effect, the survival of cardiac allografts to rats which received a single DST was examined. The cardiac allograft survival was found to be prolonged when the DST was performed 1 to 6 weeks before grafting. In addition, recipient rat sera collected 1 to 6 weeks after a single DST showed significant inhibition of a mixed lymphocyte reaction (MLR). This MLR inhibition correlated with prolongation of survival of histoincompatible rat cardiac allografts. It thus appears that a single DST given from 1 to 6 weeks before transplantation has a beneficial effect on allograft survival and that MLR inhibition may be essential for inducing the effect of transfusion on organ transplantation.</p>
キーワード donor-specific blood transfusion cardiac allograftingm mixed lymphocyte reaction
Amo Type Article
発行日 1988-02
出版物タイトル Acta Medica Okayama
42巻
1号
出版者 Okayama University Medical School
開始ページ 31
終了ページ 36
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 2966542
Web of Science KeyUT A1988M237800005
JaLCDOI 10.18926/AMO/31746
フルテキストURL fulltext.pdf
著者 Shiozaki, Shigehiro| Sakagami, Kenichi| Orita, Kunzo|
抄録 <p>We administered serum fractions obtained from cancer patients by double-filtration plasmapheresis (DFPP) to cancer-bearing mice to examine the effects on tumor growth and metastasis. Fraction 1 (whole plasma), fraction 2 (a plasma fraction containing substances with higher particle size), fraction 3 (a plasma fraction containing substances with smaller particle size) and saline were administered intravenously to cancer-bearing mice for 10 days following the inoculation of tumor cells. The tumor growth and metastasis in mice administered fraction 2 was far more rapid than that in the control mice. On the other hand, tumor growth in mice administered fraction 3 was significantly delayed compared with that in mice injected with fraction 2. These results suggest that factors in the higher particle-size fraction of cancer patients' sera promote the growth and the metastasis of tumors in mice, and that DFPP, which remove these factors, is an effective therapy against cancer.</p>
キーワード doble-filtration plasmapheresis immunosuppressive factors cancer thrapy
Amo Type Article
発行日 1987-04
出版物タイトル Acta Medica Okayama
41巻
2号
出版者 Okayama University Medical School
開始ページ 63
終了ページ 69
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 3591451
Web of Science KeyUT A1987H040200003
JaLCDOI 10.18926/AMO/31570
フルテキストURL fulltext.pdf
著者 Matsuoka, Junji| Sakagami, Kenichi| Fujiwara, Toshiyoshi| Onoda, Tadashi| Idani, Hitoshi| Gochi, Akira| Orita, Kunzo|
抄録 <p>A sustained release system for interleukin-2 (IL-2), and IL-2 mini-pellet (IL-2 mp), was developed by fusing IL-2 into a needle shaped collagen. Serum concentration of IL-2 after a single subcutaneous injection of the IL-2 mp into C57BL/6 mice remained elevated longer than after an injection of aqueous IL-2. IL-2 in the serum became undetectable by 6h after a subcutaneous injection of 1 x 10(6) unit of IL-2 in phosphate-buffered saline (PBS). In contrast, after a single subcutaneous injection of IL-2 mp containing the same amount of IL-2, the concentration of IL-2 increased to its maximum at 6h after injection, then began to decrease gradually. IL-2 was detected even on the third day after a single subcutaneous injection of one IL-2 mp. Augmentation of NK activity and generation of IL-2 activated killer cells were observed in the spleen from day 1--day 3 after a single subcutaneous injection of IL-2 mp into C57BL/6 mice. This activation was not observed following a single subcutaneous injection of the same amount of IL-2 in PBS. Adoptive immunotherapy by a single subcutaneous injection of IL-2 mp followed by intravenous injections of in vitro cultured IL-2 activated killer cells showed better results in decreasing the number of metastases of Lewis lung carcinoma in C57BL/6 mice than immunotherapy using IL-2 solution.(ABSTRACT TRUNCATED AT 250 WORDS)</p>
キーワード IL-2 drug delivery system immunotherapy mouse
Amo Type Article
発行日 1993-04
出版物タイトル Acta Medica Okayama
47巻
2号
出版者 Okayama University Medical School
開始ページ 79
終了ページ 84
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8506753
Web of Science KeyUT A1993LA45200002
JaLCDOI 10.18926/AMO/30317
フルテキストURL fulltext.pdf
著者 Watanabe, Akiharu| Obata, Takahiro| Nagashima, Hideo| Sakagami, Kenichi| Orita, Kunzo|
抄録 <p>A 26-year-old male with renal allograft, who received immunosuppressive treatment with azathioprine, presented marked elevations of serum biliary tract enzymes, such as gamma-glutamyl transpeptidase (5,609 units/l) and alkaline phosphatase (60.5 Bessey-Lowry units), 14 months after transplantation. Two months later the patient became icteric; he died of respiratory failure 19 months after the renal allograft. Postmortem examination revealed intrahepatic cholestasis with minimal inflammatory cell infiltration, indicating drug hepatotoxicity.</p>
キーワード renal transplantation ?-GTP intrahepatic cholestasis liver injury azathioprine
Amo Type Article
発行日 1984-12
出版物タイトル Acta Medica Okayama
38巻
6号
出版者 Okayama University Medical School
開始ページ 533
終了ページ 539
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6151783
Web of Science KeyUT A1984TX98000006
JaLCDOI 10.18926/AMO/31013
フルテキストURL fulltext.pdf
著者 Osaki, Toshihide| Sakagami, Kenichi| Orita, Kunzo|
抄録 <p>Interleukin-2 (IL2) is the obligatory signal for both T cell mitogenesis and in vitro generation of alloreactive cytotoxic T lymphocytes (CTL). An investigation was made to determine whether an antibody directed against IL2 would suppress the rejection reaction of rat cardiac allografts. Rabbit anti-interleukin 2 (anti-IL2) antiserum was obtained by immunizing at 2 week intervals over a period of 8 weeks with 10(6) U of recombinant human IL2 along with complete Freund's adjuvant. The bioassay for inhibition of IL2 activity by anti-IL2 antiserum was carried out in conjunction with the IL2-dependent cytotoxic T cell (CTLL cell) assay. Cardiac allografts of F344 rats were heterotopically transplanted into ACI rats. Seven daily doses of 1 ml of anti-IL2 antiserum were administered intravenously following transplantation. IL2-driven [3H]thymidine incorporation in CTLL cells was significantly inhibited by rabbit anti-IL2 antiserum. Graft survival in the anti-IL2 serum-treated group was significantly prolonged in a dose-dependent fashion compared to control groups. In conclusion, these results indicate that rabbit anti-IL2 antiserum may prove to be of significant value as an immunosuppressive agent in clinical organ transplantation.</p>
キーワード anti-interleukin 2 antiserum rat cardiac allograft immunosuppressive agent
Amo Type Article
発行日 1988-04
出版物タイトル Acta Medica Okayama
42巻
2号
出版者 Okayama University Medical School
開始ページ 77
終了ページ 81
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 3291556
Web of Science KeyUT A1988N237200004
JaLCDOI 10.18926/AMO/32678
フルテキストURL fulltext.pdf
著者 Sakagami, Kenichi| Saito, Shinya| Shiozaki, Shigehiro| Fujiwara, Takuzo| Haisa, Minoru| Niguma, Takefumi| Kusaka, Satoshi| Uda, Masashi| Matsuno, Tsuyoshi| Takasu, Shinji| Yerdel, Mehmet Ali| Matsuoka, Junji| Tanaka, Shinichiro| Orita, Kunzo|
抄録 <p>One-hundred-nine HLA-haploidentical living related renal transplants have been retrospectively analysed to compare the effect of donor-specific blood transfusion (DST) and different immunosuppressive regimens on graft survival and acute rejection. The recipients were divided into four groups according to the immunosuppressive therapy. Group 1 (n = 44): conventional therapy with posttransplant azathioprine (AZP) + methylprednisolone (MP). Group 2 (n = 25): pretransplant DST + posttransplant AZP + MP. Group 3 (n = 12): triple-drug therapy with posttransplant AZP + MP + cyclosporine (CS). Group 4 (n = 25): pretransplant DST + posttransplant AZP + MP + CS. The five-year actuarial survival rates for groups 1, 2, 3 and 4 were 48%, 73%, 79%, and 89%, respectively. The graft survival rate in group 3 was significantly (p less than 0.01) better than that in group 1. The transfusion effect was reduced, and appears as a 10% improvement in the graft survival in the cyclosporin era compared with a 25% improvement at pre-cyclosporin era. Furthermore, the incidence of the first rejection episode was decreased in recipients that received DST. The present study revealed that DST, as pretransplant conditioning has a definite impact on rejection-free long-term graft survival in HLA-haploidentical living-related kidney recipients and the most favorable outcome in such patients could be achieved by DST pretreatment in conjunction with posttransplant triple-drug therapy including cyclosporine.</p>
キーワード living-related kindney transplantation donor-specific blood transfusion (DST) cyclosporine
Amo Type Article
発行日 1992-02
出版物タイトル Acta Medica Okayama
46巻
1号
出版者 Okayama University Medical School
開始ページ 1
終了ページ 5
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 1561899
Web of Science KeyUT A1992HH01700001
JaLCDOI 10.18926/AMO/31998
フルテキストURL fulltext.pdf
著者 Nakao, Atsunori| Sakagami, Kenichi| Mitsuoka, Shintaro| Uda, Masashi| Tanaka, Noriaki|
抄録 <p>We report a case of retroperitoneal hematoma presenting as femoral nerve pulsy on antiplatelet therapy. The patient, a 78-year-old man who had undergone antiplatelet treatment using ticlopidine, was admitted to our hospital with complaints of sudden-onset low abdominal and back pain. Computed tomography showed an iso-density mass in the right retroperitoneum within the psoas muscle. We made a diagnosis of retroperitoneal hematoma compressing the femoral nerve and performed an operation to remove the hematoma in order to decompress the femoral neuropathy. Postoperatively, the patient rapidly recovered from the femoral neuropathy. In the particular case in which no antagonist against the ticlopidine is available, surgical decompression could produce a good outcome.</p>
キーワード ticlopidine retroperitoneal hematoma
Amo Type Article
発行日 2001-12
出版物タイトル Acta Medica Okayama
55巻
6号
出版者 Okayama University Medical School
開始ページ 363
終了ページ 366
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 11779099
Web of Science KeyUT 000172838400007
JaLCDOI 10.18926/AMO/31581
フルテキストURL fulltext.pdf
著者 Saito, Shinya| Sakagami, Kenichi| Fujiwara, Takuzo| Matsuno, Tsuyoshi| Orita, Kunzo| Hiramatsu, Yuji| Kudo, Takafumi|
抄録 <p>Three cases of successful pregnancies in renal transplant recipients who had undergone transplantation in the Okayama University Medical School Hospital are reported. Two of the women had received an organ from a living relative and one woman received a cadaveric organ graft. These patients, aged 28-37 at the time of the delivery, had received their transplants 2-5 years prior to their conception. The periods of gestation ranged between 35 and 40 weeks. The weight of the babies at birth ranged from 2,380g to 2,500g and the apgar score at 1 min was 8 or 9. None of the infants showed any congenital abnormalities. Lower-segment cesarean section was performed in all of three cases. Serum creatinine levels, an indicator of renal graft function, did not deteriorate during the pregnancy or after delivery. Although further work is needed to solve problems regarding pregnancy in renal transplant recipients, these results encouraged us to meet their hope for a baby.</p>
キーワード pregnancy renal transplantation immunosuppression
Amo Type Article
発行日 1993-10
出版物タイトル Acta Medica Okayama
47巻
5号
出版者 Okayama University Medical School
開始ページ 347
終了ページ 349
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8273458
Web of Science KeyUT A1993ME47100009
JaLCDOI 10.18926/AMO/32683
フルテキストURL fulltext.pdf
著者 Sakagami, Kenichi| Saito, Shinya| Shiozaki, Shigehiro| Takasu, Shinji| Matsuno, Tsuyoshi| Fujiwara, Takuzo| Kusaka, Satoshi| Uda, Masashi| Matsuoka, Junji| Naomoto, Yoshio| Gouchi, Akira| Hamazaki, Keisuke| Tanaka, Shinichiro| Orita, Kunzo|
抄録 <p>A retrospective study was carried out in 110 cadaveric kidney transplant recipients to compare the effects of low doses of cyclosporine (CsA), azathioprine (AZP) and steroids (triple-drug therapy) with those of higher doses of steroids plus AZP (conventional immunosuppression). Graft survival rate in the triple-drug therapy was 77%, 69%, and 69% at 1, 3, and 5 years, respectively. This was significantly better than 48%, 34%, and 29% in conventional immunosuppression. The incidence of acute rejection episodes was significantly lower in the triple-drug therapy than in conventional immunosuppression (25% vs 58%). In conclusion, our study shows that triple-drug therapy using low-dose cyclosporine is the safest of the immunosuppressive regimens and provides a beneficial effect on the long-term survival of cadaveric kidney transplants.</p>
キーワード cadaveric kindney transplantation cyclosporine triple-drug therapy
Amo Type Article
発行日 1992-02
出版物タイトル Acta Medica Okayama
46巻
1号
出版者 Okayama University Medical School
開始ページ 53
終了ページ 56
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 1561907
Web of Science KeyUT A1992HH01700010
JaLCDOI 10.18926/AMO/31132
フルテキストURL fulltext.pdf
著者 Fujiwara, Takuzo| Sakagami, Kenichi| Saito, Shinya| Uda, Masashi| Orita, Kunzo|
抄録 <p>The antidonor immune response was examined in a one haplotype-mismatched renal transplant recipient with an allograft that had been well-functioning for more than 10 years. Although the relative response of the mixed lymphocyte reaction (MLR) was (45.8)% and the MLR responder cells stimulated by donor cells produced measurable amounts of interleukin-2 (IL-2) (11.6 U/ml), the cytotoxic T lymphocytes (CTL) could not be generated against donor cells, even with exogenous IL-2. These results indicate that antidonor CTL precursors were either deleted or inactivated in this recipient.</p>
キーワード renal transplantation long-term stable recipient cytotoxic T lymphocytes
Amo Type Article
発行日 1994-02
出版物タイトル Acta Medica Okayama
48巻
1号
出版者 Okayama University Medical School
開始ページ 63
終了ページ 65
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8191919
Web of Sience KeyUT A1994MY85100011