JaLCDOI 10.18926/AMO/31820
フルテキストURL fulltext.pdf
著者 Miyake, Kohei| Nishida, Keiichiro| Kadota, Yasutaka| Yamasaki, Hiroko| Nasu, Tatsuyo| Saitou, Daisuke| Tanabe, Katsuyuki| Sonoda, Hikaru| Sato, Yasufumi| Maeshima, Yohei| Makino, Hirofumi|
抄録 <p>Angiogenesis is an essential event in the development of synovial inflammation in rheumatoid arthritis (RA). The aim of the current study was to investigate the expression of vasohibin-1, a novel endothelium-derived vascular endothelial growth factor (VEGF)-inducible angiogenesis inhibitor, in the RA synovium, and to test the effect of inflammatory cytokines on the expression of vasohibin-1 by RA synovial fibroblasts (RASFs). Synovial tissue samples were obtained at surgery from patients with osteoarthritis (OA) and RA, and subjected to immunohistochemistry to investigate the expression and distribution of vasohibin-1 relevant to the degree of synovial inflammation. In an in vitro analysis, RASFs were used to examine the expression of vasohibin-1 and VEGF mRNA by real-time PCR after stimulation with VEGF or inflammatory cytokines under normoxic or hypoxic conditions. The immunohistochemical results showed that vasohibin-1 was expressed in synovial lining cells, endothelial cells, and synovial fibroblasts. In synovial tissue, there was a significant correlation between the expression of vasohibin-1 and histological inflammation score (p0.002, r0.842). In vitro, stimulation with VEGF induced the expression of vasohibin-1 mRNA in RASFs under normoxic conditions, and stimulation with cytokines induced vasohibin-1 mRNA expression under a hypoxic condition. These results suggest that vasohibin-1 was expressed in RA synovial tissue and might be regulated by inflammatory cytokines.</p>
キーワード angiogenesis vasohibin-1 rheumatoid arthritis synovial membrane VEGF
Amo Type Original Article
発行日 2009-12
出版物タイトル Acta Medica Okayama
63巻
6号
出版者 Okayama University Medical School
開始ページ 349
終了ページ 358
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 20035291
Web of Sience KeyUT 000273145900006