JaLCDOI 10.18926/AMO/32644
フルテキストURL fulltext.pdf
著者 Saito, Chisato| Nishioka, Keiko| Ishii, Akira| Ohata, Nobuo| Masuda, Yu|
抄録 <p>Using 6 fractions differing in molecular weight of Dermatophagoides pteronyssinus (Dp)-antigen, we measured by enzyme-linked immunosorbent assay (ELISA) the titers of specific IgE, IgG and IgG4 antibodies against Dp antigen in sera of allergic subjects who were sensitive to house dust mite. We intended to evaluate which Dp fraction acts as the major antigenicity for allergic subjects. Results were as follows: 1) In comparison with normal controls, the titer of IgE antibody specific to crude Dp antigen was evaluated, but no significant difference was found among the titers of IgE antibody against each Dp fraction. 2) The titer of IgG antibody against the fraction with a high molecular weight (190 KD, 95 KD) was significantly higher than the titer of the 15 KD fraction in the nasal allergy patients. 3) The 15 KD fraction induced significant elevation of the titer of IgG4 antibody. It suggests that the low molecular weight fraction may act as the major allergenicity of Dp-antigen for inducing both IgE and competitive IgG4 antibodies, although other fractions induce significant IgE responses in patients with nasal allergy.</p>
キーワード IgG4 IgE nasal allergy Dermatophagoides pteronyssinus
Amo Type Article
発行日 1992-04
出版物タイトル Acta Medica Okayama
46巻
2号
出版者 Okayama University Medical School
開始ページ 93
終了ページ 101
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 1575063
Web of Sience KeyUT A1992HR48400006
JaLCDOI 10.18926/AMO/31594
フルテキストURL fulltext.pdf
著者 Nishioka, Keiko| Meguro, Tadamichi| Okano, Mitsuhiro| Masuda, Yu| Saito, Chisato| Yasumitsu, Eiji|
抄録 <p>Fifteen patients with Japanese cedar pollenosis were examined for lower airway function. Flow-volume patterns obtained from flow-volume and volume-time curves during the pollen season (March) and outside of the pollen season (June) were evaluated. In a previous report we classified maximal expiratory flow-volume (MEFV) curves in five patterns from A to E. In the present study, the patterns did not vary between the two periods except in one patient. Eleven patients out of 15 showed type E patterns, in which the flow-volume curve was concave along its entire course. In most of the patients with severe or moderate symptoms of allergic rhinitis only during the pollen season, the curve shifted to the right, but the parameters of the curves did not increase significantly outside of season. These findings suggest that patients with Japanese cedar pollenosis suffer from continuous latent peripheral airway obstruction. Extremely slight changes in the flow rate were detected by comparing the curves obtained during the two periods.</p>
キーワード pulmonary function test pollenosis pollinosis maximal expiratory flow-volume curve flowvolume pattern
Amo Type Article
発行日 1993-06
出版物タイトル Acta Medica Okayama
47巻
3号
出版者 Okayama University Medical School
開始ページ 151
終了ページ 156
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8379343
Web of Sience KeyUT A1993LL12400003
JaLCDOI 10.18926/AMO/31119
フルテキストURL fulltext.pdf
著者 Nishioka, Keiko| Saito, Chisato| Nagano, Toshiaki| Okano, Mitsuhiro| Nakagawa, Fumio| Nishioka, Satoko| Masuda, Yu| Ono, Toshiro|
抄録 <p>To determine the allergens of mite allergic rhinitis, we studied 31 patients with mite allergic rhinitis by skin tests and nasal provocation tests (15 for skin and 16 for nasal tests) using 6 fractions of Dermatophagoides pteronyssinus (Dp) extract differing in molecular weights (15, 25, 32, 53, 95 and 190 kDMW). In skin testing, patients showed intense positive reactions to the fractions of 15, 25, 32, 95 and 190 kDMW, among which the most patients showed positive reactions to the fractions of 15 and 25 kDMW. Significant differences were found in patients' positive reactivity among each fraction and between low (15 and 25 kD) and high (95 and 190 kD) molecular weight fractions as well. In nasal provocation tests, patients showed intense positive reactions to the fractions of 15, 32, 53 and 95 kDMW, especially to the fractions of 15 and 95 kDMW. Furthermore, the insidence of positive reactions to the 15 kDMW fraction was significantly higher than that to any other fraction in the skin tests (P &#60; 0.05). From these results, the low molecular weight fraction, 15 kDMW, is considered to be the main allergen of this mite and the high molecular weight fractions, 95 and 190 kDMW, may also be considered to be allergens of this mite.</p>
キーワード mite allergen skin test nasal provoration test allergen rhinitis
Amo Type Article
発行日 1994-10
出版物タイトル Acta Medica Okayama
48巻
5号
出版者 Okayama University Medical School
開始ページ 279
終了ページ 282
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 7863799
Web of Sience KeyUT A1994PP23600008
JaLCDOI 10.18926/AMO/31114
フルテキストURL fulltext.pdf
著者 Nishioka, Keiko| Saito, Chisato| Nagano, Toshiaki| Okano, Mitsuhiro| Nakagawa, Fumio| Nishioka, Satoko| Masuda, Yu| Ono, Toshiro|
抄録 <p>To determine the allergens of mite allergic rhinitis, we studied 31 patients with mite allergic rhinitis by skin tests and nasal provocation tests (15 for skin and 16 for nasal tests) using 6 fractions of Dermatophagoides pteronyssinus (Dp) extract differing in molecular weights (15, 25, 32, 53, 95 and 190 kDMW). In skin testing, patients showed intense positive reactions to the fractions of 15, 25, 32, 95 and 190 kDMW, among which the most patients showed positive reactions to the fractions of 15 and 25 kDMW. Significant differences were found in patients' positive reactivity among each fraction and between low (15 and 25 kD) and high (95 and 190 kD) molecular weight fractions as well. In nasal provocation tests, patients showed intense positive reactions to the fractions of 15, 32, 53 and 95 kDMW, especially to the fractions of 15 and 95 kDMW. Furthermore, the insidence of positive reactions to the 15 kDMW fraction was significantly higher than that to any other fraction in the skin tests (P &#60; 0.05). From these results, the low molecular weight fraction, 15 kDMW, is considered to be the main allergen of this mite and the high molecular weight fractions, 95 and 190 kDMW, may also be considered to be allergens of this mite.</p>
キーワード mite allergen skin test nasal provoration test allergen rhinitis
Amo Type Article
発行日 1994-10
出版物タイトル Acta Medica Okayama
48巻
5号
出版者 Okayama University Medical School
開始ページ 279
終了ページ 282
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 7863799
Web of Sience KeyUT A1994PP23600008
JaLCDOI 10.18926/AMO/30420
フルテキストURL fulltext.pdf
著者 Nishioka, Keiko| Ogawa, Teruhiro| Saito, Chisato| Nishioka, Satoko| Nakagawa, Fumio| Ohomichi, Takuya| Masuda, Yu|
抄録 <p>In 15 patients with Japanese cedar pollinosis and 10 healthy control subjects, levels of major basic protein (MBP), eosinophil cationic protein (ECP), and arylsulfatase B (As) in the nasal secretions were examined before and after challenge with Japanese cedar pollen extract. The MBP and ECP levels in the patients were significantly higher 30 min after challenge than those before challenge (P &#60; 0.005). MBP and ECP levels after challenge were significantly higher in the nasal secretions of patients than in the controls (MBP: P &#60; 0.01, ECP: P &#60; 0.05). The level of As after challenge was significantly higher in the nasal secretions of patients than in the controls. These results suggest that eosinophils activate or modify the immediate, nasal allergic reaction and have a role in regulating immunological responses.</p>
キーワード major basic protein eosinophil cationic protein arylsulphatase B pollinosis allergic rhinitis
Amo Type Article
発行日 1995-02
出版物タイトル Acta Medica Okayama
49巻
1号
出版者 Okayama University Medical School
開始ページ 29
終了ページ 33
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 7762407
Web of Sience KeyUT A1995QK32500005