JaLCDOI 10.18926/AMO/30709
フルテキストURL fulltext.pdf
著者 Okada, Soji| Miyai, Yooichiro| Ofuji, Tadashi|
抄録 <p>The patient was a 37-year-old female teacher with hyperemesis diabeticorum and juvenile Type-I diabetes. At the age of 29 years, nausea and vomiting developed and secured at nearly weekly intervals. She was started on clotiazepam (15 mg/day). The vomiting was cured and psychological improvement was evident; her anxiety about diabetes was markedly reduced. An X-ray examination after the administration of clotiazepam showed that she was entirely free from marked hypoperistalsis and the severe retention of gastric contents which had been present before this treatment. The present case is a clear example of stress closely related to the pathogenesis of hyperemesis diabeticorum.</p>
キーワード antianxiety drug hyperemesis diabeticorum
Amo Type Brief Note
発行日 1982-02
出版物タイトル Acta Medica Okayama
36巻
1号
出版者 Okayama University Medical School
開始ページ 81
終了ページ 84
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6121462
Web of Science KeyUT A1982NE20000010
JaLCDOI 10.18926/AMO/31274
フルテキストURL fulltext.pdf
著者 Makino, Hirofumi| Takaoka, Michio| Komoda, Keizo| Tokuyama, Kiyoyuki| Ota, Zensuke| Ofuji, Tadashi| Kaneshige, Tetsuji|
抄録 <p>Masugi nephritis was induced in rats by a single intravenous injection of rabbit anti-rat kidney serum, and studied with a scanning electron microscope. A characteristic finding was the presence of white cells, probably polymorphonuclear leukocytes, with many microspikes which penetrated through degenerated glomerular endothelial cells to be in direct contact with the glomerular basement membrane. This finding confirms the pathogenic role of leukocytes in glomerulonephritis induced by anti-glomerular basement membrane antibody.</p>
キーワード Masugi nephritis scanning electron microscopy leukocyte.
Amo Type Brief Note
発行日 1981-11
出版物タイトル Acta Medica Okayama
35巻
5号
出版者 Okayama University Medical School
開始ページ 373
終了ページ 376
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6459000
Web of Sience KeyUT A1981MS42400007
JaLCDOI 10.18926/AMO/31275
フルテキストURL fulltext.pdf
著者 Asano, Taro| Yano, Keisuke| Ofuji, Tadashi|
抄録 <p>To investigate cellular interactions between human T and B lymphocytes in various diseases, we established a technique to prove terminal differentiation of B lymphocytes into immunoglobulin synthesizing and secreting cells. We also established a double antibody radioimmunoassay to measure the amount of IgG, IgA and IgM synthesized and secreted in culture supernatants. Purified immunoglobulins were obtained from sera of patients with myeloma or macroglobulinemia. The peripheral blood lymphocytes from 25 normal individuals had the geometric mean synthetic rates of 1886 ng for IgG, 1607 ng for IgA and 1173 ng for IgM per 1 X 10(6) cells when cultured for nine days in the presence of pokeweed mitogen. The method is simple and sensitive, and is thought to be useful for examining human lymphocyte function in vitro.</p>
キーワード lymphocytes PWM immunoglobulins RIA.
Amo Type Article
発行日 1981-11
出版物タイトル Acta Medica Okayama
35巻
5号
出版者 Okayama University Medical School
開始ページ 319
終了ページ 326
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6458995
Web of Sience KeyUT A1981MS42400002
JaLCDOI 10.18926/AMO/31291
フルテキストURL fulltext.pdf
著者 Ohno, Norihito| Hashimoto, Kozo| Yunoki, Sho| Takahara, Jiro| Ofuji, Tadashi|
抄録 <p>Chromosome studies were conducted on two patients with adult T-cell leukemia. In both patients, a marker chromosome 14q+ and a structural change involving chromosome 1 with trisomy of the q arm were found in peripheral blood leukocytes. The 14q+ marker chromosome had resulted from translocation from #5p in one patient and #5q in the other patient. The present and previous studies suggest that the donor chromosomes involved in the 14q+ translocation are variable. This indicates that the 14q+ marker chromosome rather than the donor chromosome is intimately related with adult T-cell leukemia.</p>
Amo Type Article
発行日 1981-02
出版物タイトル Acta Medica Okayama
35巻
1号
出版者 Okayama University Medical School
開始ページ 19
終了ページ 25
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6455044
Web of Science KeyUT A1981LH76300002
JaLCDOI 10.18926/AMO/31293
フルテキストURL fulltext.pdf
著者 Hashimoto, Kozo| Ohno, Norihito| Aoki, Yoshiyuki| Kageyama, Jingo| Takahara, Jiro| Ofuji, Tadashi|
抄録 <p>The distribution of corticotropin releasing factor (CRF) and arginine vasopressin (AVP) in hypothalamic nuclei were examined in control and estrogen-treated female rats. CRF activity was measured using monolayer cultured rat anterior pituitary cells and AVP by radioimmunoassay. Hypothalamic nuclei were punched out according to the method of Palkovits. The distribution of CRF activity in 5 different hypothalamic nuclei was similar to that of AVP in intact female rats. CRF activity in hypothalamic nuclei, pituitary ACTH content and plasma ACTH levels in estrogen-treated rats were not significantly different from those in control rats. However, significant elevation of AVP content was observed in the supraoptic and paraventricular nuclei of estrogen-treated rats. These results indicate that CRF and AVP are distributed in similar hypothalamic nuclei, but that they are not identical.</p>
キーワード corticotropin releasing factor (CRF) arginine vasopressin (AVP) hypothalamic nucler.
Amo Type Article
発行日 1981-02
出版物タイトル Acta Medica Okayama
35巻
1号
出版者 Okayama University Medical School
開始ページ 37
終了ページ 43
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6266205
Web of Sience KeyUT A1981LH76300004
JaLCDOI 10.18926/AMO/31290
フルテキストURL fulltext.pdf
著者 Hashimoto, Kozo| Ohno, Norihito| Aoki, Yoshiyuki| Kageyama, Jingo| Takahara, Jiro| Ofuji, Tadashi|
抄録 <p>The distribution of corticotropin releasing factor (CRF) and arginine vasopressin (AVP) in hypothalamic nuclei were examined in control and estrogen-treated female rats. CRF activity was measured using monolayer cultured rat anterior pituitary cells and AVP by radioimmunoassay. Hypothalamic nuclei were punched out according to the method of Palkovits. The distribution of CRF activity in 5 different hypothalamic nuclei was similar to that of AVP in intact female rats. CRF activity in hypothalamic nuclei, pituitary ACTH content and plasma ACTH levels in estrogen-treated rats were not significantly different from those in control rats. However, significant elevation of AVP content was observed in the supraoptic and paraventricular nuclei of estrogen-treated rats. These results indicate that CRF and AVP are distributed in similar hypothalamic nuclei, but that they are not identical.</p>
キーワード corticotropin releasing factor (CRF) arginine vasopressin (AVP) hypothalamic nucler.
Amo Type Article
発行日 1981-02
出版物タイトル Acta Medica Okayama
35巻
1号
出版者 Okayama University Medical School
開始ページ 37
終了ページ 43
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6266205
Web of Sience KeyUT A1981LH76300004
JaLCDOI 10.18926/AMO/31292
フルテキストURL fulltext.pdf
著者 Ohno, Norihito| Hashimoto, Kozo| Yunoki, Sho| Takahara, Jiro| Ofuji, Tadashi|
抄録 <p>A perifusion method has been developed using rat hypothalamo-neurohypophyseal system (HNS) or neural lobe to investigate the control mechanism of arginine vasopressin (AVP) release. A specific radioimmunoassay (RIA) for AVP was developed to measure AVP in perifusion medium employing anti-AVP serum which was obtained by immunizing rabbits. At a final dilution of 1/12,000, the antiserum showed less than 0.66 and 0.01% cross reactivity with lysine-vasopressin and oxytocin, respectively. But it did not cross reacted with other peptide hormones. The lowest detectable level of vasopressin was 0.5 pg/tube. The intra-assay coefficient of variation averaged 10.4%. The dilution curve of perifused medium was well paralled to the standard curve of AVP assay. AVP release from HNS or neural lobe gradually declined to the stable level in 90-120 min after the initiation of perifusion. Good repeatability of the AVP release from neural lobe was recognized by repeated stimulation with 10 min perifusion of 60 mM KCl at every 60 min. HNS released AVP in dose related manner to the osmotic challenge of sodium or glucose, and AVP release was stimulated from HNS by prostaglandin E2, but not by dopamine. These results show that the perifusion methods using AVP-RIA is a useful method to examine the AVP release from HNS or neural lobe.</p>
Amo Type Article
発行日 1981-02
出版物タイトル Acta Medica Okayama
35巻
1号
出版者 Okayama University Medical School
開始ページ 27
終了ページ 35
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6455045
Web of Science KeyUT A1981LH76300003
JaLCDOI 10.18926/AMO/30561
フルテキストURL fulltext.pdf
著者 Miyoshi, Masanori| Nakashima, Yusaku| Hirakawa, Shuzo| Mitsunaga, Mikio| Nakagawa, Osamu| Miura, Hiroshi| Suzuki, Shinya| Ofuji, Tadashi|
抄録 <p>Function of pituitary-thyroid axis was studied in rats with experimentally induced thyroiditisWistar strain female rats were immunized with homologous thyroid extract in Freund's complete adjuvant and with simultaneous intradermal injection of pertussis vaccine concentrate. They received booster shots at the first and third week after the initial immunization. Serum thyroid hormones and TSH were measured just before, and at weekly intervals after, the initial immunization. Histological examination of the thyroid gland at the second week after immunization showed slight infiltration of macrophages in the thyroid follicles. From the third to the fourth week, massive lymphoid cell infiltration and destruction of follicular architecture developed in all immunized rats. Serum R3 levels slightly decreased during the second week, increased transiently during the third week, then decreased again thereafter. Serum T4 levels decreased slightly durinf the fourth week. Serum TSH levels were not elevated significantly during the third week, but the response to TRH was significantly increased at this time. Basal TSH levels were increased during the fourth week. The TRH test was a sensitive method capable of detecting minimal failure of thyroid function undetected by other routine measuremens of thyroid hormones and TSH.</p>
キーワード thyroid function thyrotropin (TSH) experimental thyroiditis thyroid hormones.
Amo Type Article
発行日 1980-02
出版物タイトル Acta Medica Okayama
34巻
1号
出版者 Okayama University Medical School
開始ページ 11
終了ページ 17
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6446835
Web of Science KeyUT A1980JS13800002
JaLCDOI 10.18926/AMO/30562
フルテキストURL fulltext.pdf
著者 Takaya, Yasumasa| Ota, Zensuke| Makino, Hirofumi| Kida, Keiko| Miyoshi, Akira| Hiramatsu, Makoto| Takahashi, Kayo| Ofuji, Tadashi|
抄録 Isolated and purified glomerular basement membranes (GBM) of normal and aminonucleoside (PAN) nephrosis rats were observed by electron microscopy after negative staining. Although GBM of normal rats appeared as a molecular sieve with uniform pores, GBM of nephrotic rats showed enlargement and elongation of the pores. For an average of fifty pores, the long dimension was 40.4+/-10.7 A and the short dimension 13.8+/-3.6 A in nephrosis whereas the long dimension was 12.3+/-2.5 A and the short dimension 8.4+/-1.0 A in normal rats. Changes in the pores in GBM were thought to result in increased permeability of serum protein and hence proteinuria.
キーワード gromerular basement membranc aminonucleoside nephrosis negative staining.
Amo Type Brief Note
発行日 1980-02
出版物タイトル Acta Medica Okayama
34巻
1号
出版者 Okayama University Medical School
開始ページ 67
終了ページ 70
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6446842
Web of Science KeyUT A1980JS13800009
JaLCDOI 10.18926/AMO/32035
フルテキストURL fulltext.pdf
著者 Miyoshi, Akira| Makino, Hirofumi| Hiramatsu, Makoto| Takahashi, Kayo| Yamane, Yukio| Takahara, Jiro| Suzuki, Shinya| Ota, Zensuke| Ofuji, Tadashi| Takehisa, Yoshiaki|
抄録 <p>A 26-year-old female with Bartter's syndrome associated with Graves' disease is reported. This patient had a history of Graves' disease from the age of 22 and anti-thyroid drug (Methimazole) had been administered for 2 years. Thyroid function returned to normal but general fatigue and polyuria continued. Hypokalemia was diagnosed at 25 years of age and she was referred to our hospital for evaluation. Blood pressure was normal and laboratory data revealed normal thyroid function, hypokalemic alkalosis, high plasma renin activity and high plasma aldosterone concentration. She showed normal pressor sensitivity to norepinephrine infusion, grossly diminished pressor sensitivity to exogenous angiotensin II infusion compared with the normal. A renal biopsy specimen showed juxtaglomerular cell hyperplasia. Electron microscopy confirmed lacis cell (agranular cell) proliferation.</p>
キーワード Bartter's syndrome juxtaglomerular cell hyperplasia secondary aldosteronism plasma renin activity
Amo Type Article
発行日 1979-08
出版物タイトル Acta Medica Okayama
33巻
4号
出版者 Okayama University Medical School
開始ページ 305
終了ページ 314
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 158951
JaLCDOI 10.18926/AMO/32041
フルテキストURL fulltext.pdf
著者 Yokoyama, Hisamitsu| Yumane, Yukio| Takahara, Jiro| Yoshinouchi, Takeo| Ofuji, Tadashi|
抄録 <p>An unusual case of orbital tumor with high renin content and severe hypertension is described. The patient was a 15-year-old girl with juvenile hypertension (200-140 mmHg) associated with right exophthalmos and hypokalemia. The patient showed extremely high levels of plasma renin activity and plasma aldosterone concentration. No difference was present in plasma renin activity from either side of the renal veins. Preoperatively, hypertension responded to treatment with spironolactone. The tumor could not be completely removed because of intracranial metastasis and infiltration, and the hyperreninemia and secondary hyperaldosteronism persisted. The renin content in the orbital tissue was 1,403-2,225 ng/angiotensin I generated/h/g wet weight of tissue. The postmortem histopathologic diagnosis was orbital hemangiopericytoma. This is the first case of extrarenal (ectopic) renin-secreting (or -producing) hemangiopericytoma of the orbital origin. Furthermore this case is worthy of note in the point of view of the presence of the extrarenal renin-angiotensin system, particularly in the brain.</p>
キーワード renin producing tumor hemangiopericytoma juvenile hypertension hypokalemia orbital tumor
Amo Type Brief Note
発行日 1979-08
出版物タイトル Acta Medica Okayama
33巻
4号
出版者 Okayama University Medical School
開始ページ 315
終了ページ 322
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 158952
NAID 120002311859
JaLCDOI 10.18926/AMO/32039
フルテキストURL fulltext.pdf
著者 Yamana, Seizo| Nakamura, Zenichi| Saito, Yoshihito| Yamamoto, Michinori| Ofuji, Tadashi|
抄録 <p>Purification of antilymphocyte antibody (ALA) from patients with systemic lupus erythematosus (SLE) was achieved by immunoabsorption and elution. Human tonsil cells or thymocytes were used as absorbents. Complement dependent microcytotoxicity tests showed that, in comparison to the parent sera, the eluate from tonsil cells was eight times, and that from thymocytes four times, more active. Antinuclear activity was eliminated by elution. The ALA was almost entirely IgM, IgG being involved in only a few cases. IgA lacked cytotoxic activity. ALA was directed at both T- and B-cell surface determinants, which suggests that, in SLE, it has a heterogeneous biological composition.</p>
キーワード antilymphocyte antibody systemic lupus erythematosus purification
Amo Type Article
発行日 1979-08
出版物タイトル Acta Medica Okayama
33巻
4号
出版者 Okayama University Medical School
開始ページ 259
終了ページ 267
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 91309
JaLCDOI 10.18926/AMO/32052
フルテキストURL fulltext.pdf
著者 Makino, Hirofumi| Ota, Zensuke| Takaya, Yasumasa| Kida, Keiko| Miyoshi, Akira| Hiramatsu, Makoto| Takahashi, Kayo| Ofuji, Tadashi|
抄録 <p>The tubular basement membrane (TBM) (i.e. tubular basal lamina) of rat kidney was shown to be a fine meshwork by electron microscopy after negative staining. Strands of the meshwork formed a regular three dimensional lattice work. The pores of the meshwork were polygonal. There were two main pore sizes: one approximately 30 A in diameter, the other 42--60 A. In view of our previous observation that glomerular and alveolar basement membranes were made up fine meshwork, it is quite possible that the basement membranes of other organs are also made up such fine meshwork.</p>
キーワード renal tubular basement membrane mesh work structure pore negative staining electron micro scopy
Amo Type Brief Note
発行日 1979-04
出版物タイトル Acta Medica Okayama
33巻
2号
出版者 Okayama University Medical School
開始ページ 133
終了ページ 136
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 89787
NAID 120002312032
JaLCDOI 10.18926/AMO/32053
フルテキストURL fulltext.pdf
著者 Hashimoto, Kozo| Yunoki, Sho| Hosogi, Hidemi| Takahara, Jiro| Ofuji, Tadashi|
抄録 <p>Biologically active peptides and neurotransmitter substances were added to anterior pituitary cell cultures to examine the presence of corticotropin releasing factor (CRF)-like activity. Hypothalamic extract (HE) induced significant dose-related increase of ACTH, and the lowest effective dose was 0.01 HE/ml. Other tested substances including luteinizing hormone-releasing hormone, thyrotropin releasing hormone, melanocyte stimulating hormone release inhibiting factor, somatostatin, substance P, neurotensin, beta-endorphin. leu-enkephalin, met-enkephalin, bradykinin, norepinephrine, dopamine, serotonin, acetylcholine, histamine, gamma-amino butyric acid or gamma-hydroxy butyric acid showed no CRF-like activity. Relatively high doses of lysine vasopressin, arginine vasopressin and angiotensin II increased the release of ACTH in pituitary cell cultures, but the maximal ACTH response was markedly less than with HE. These results indicate that cultured anterior pituitary cells are sensitive and fairly specific in detecting CRF(s) comparing with other detecting procedures.</p>
キーワード pituitary cell cultures corticotropin releasing factor ACTH neuropeptides neurotransmitter substances
Amo Type Article
発行日 1979-04
出版物タイトル Acta Medica Okayama
33巻
2号
出版者 Okayama University Medical School
開始ページ 81
終了ページ 90
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 38634
NAID 120002311394
JaLCDOI 10.18926/AMO/32075
フルテキストURL fulltext.pdf
著者 Mitsunaga, Mikio| Hasegawa, Kan| Nakagawa, Osamu| Nakashima, Yusaku| Miyoshi, Masanori| Suzuki, Shinya| Ofuji, Tadashi|
抄録 <p>A case of a 40-year-old woman who was suffering from painless thyroiditis with recurrent transient thyrotoxicosis is reported. Acute exacerbations occurred four times during the past ten years, two after delivery and two after catching a cold. Serum thyroid hormones increased, though radioiodine uptake by the thyroid was very low and no inflammatory signs were observed. The histological findings of the thyroid were of atypical thyroiditis and not consistent with either chronic lymphocytic thyroiditis or subacute thyroiditis. Tanned sheep red cell hemagglutination titers for anti-thyroglobulin antibodies (TRC) and for anti-microsomal antibodies (MHA) were negative or low. The disease seems to be rare and the pathophysiology and etiology are discussed.</p>
キーワード recurrent transient thyrotoxicosis painless thyroiditis hyper-thyroiditis subacute thyroiditis chronic lymphocytic thyroiditis
Amo Type Article
発行日 1979-02
出版物タイトル Acta Medica Okayama
33巻
1号
出版者 Okayama University Medical School
開始ページ 43
終了ページ 51
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 155981
著者 Yamana, Seizo| Matsueda, Hideki| Ezawa, Hidemitsu| Miyake, Susumu| Katsuyama, Shunsaku| Ofuji, Tadashi|
発行日 1978-12
出版物タイトル Acta Medica Okayama
32巻
6号
資料タイプ 学術雑誌論文
著者 Sarai, Tetsuo| Kawanishi, Koichi| Saito, Yoshihito| Aoi, Katsuyuki| Nishina, Yoshiaki| Ofuji, Tadashi|
発行日 1978-08
出版物タイトル Acta Medica Okayama
32巻
4号
資料タイプ 学術雑誌論文
著者 Kawanishi, Koichi| Goto, Akio| Ishida, Toshihiko| Kawamura, Ko| Nishina, Yoshiaki| Machida, Shuji| Yamamoto, Shinro| Ofuji, Tadashi|
発行日 1978-08
出版物タイトル Acta Medica Okayama
32巻
4号
資料タイプ 学術雑誌論文
著者 Kawanishi, Koichi| Ueda, Hidenori| Nagase, Masayuki| Ofuji, Tadashi|
発行日 1977-10
出版物タイトル Acta Medica Okayama
31巻
5号
資料タイプ 学術雑誌論文
著者 Ota, Zensuke| Makino, Hirofumi| Miyoshi, Akira| Hiramatsu, Makoto| Takahashi, Kayo| Ofuji, Tadashi|
発行日 1977-10
出版物タイトル Acta Medica Okayama
31巻
5号
資料タイプ 学術雑誌論文