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ID 31275
JaLCDOI
フルテキストURL
著者
Asano, Taro Okayama University
Yano, Keisuke Okayama University
Ofuji, Tadashi Okayama University
抄録

To investigate cellular interactions between human T and B lymphocytes in various diseases, we established a technique to prove terminal differentiation of B lymphocytes into immunoglobulin synthesizing and secreting cells. We also established a double antibody radioimmunoassay to measure the amount of IgG, IgA and IgM synthesized and secreted in culture supernatants. Purified immunoglobulins were obtained from sera of patients with myeloma or macroglobulinemia. The peripheral blood lymphocytes from 25 normal individuals had the geometric mean synthetic rates of 1886 ng for IgG, 1607 ng for IgA and 1173 ng for IgM per 1 X 10(6) cells when cultured for nine days in the presence of pokeweed mitogen. The method is simple and sensitive, and is thought to be useful for examining human lymphocyte function in vitro.

キーワード
lymphocytes
PWM
immunoglobulins
RIA.
Amo Type
Article
発行日
1981-11
出版物タイトル
Acta Medica Okayama
35巻
5号
出版者
Okayama University Medical School
開始ページ
319
終了ページ
326
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT