JaLCDOI 10.18926/AMO/30891
フルテキストURL fulltext.pdf
著者 Tanaka, Juntaro| Harada, Hideo| Ochi, Koji| Miyake, Hirofumi| Kochi, Fumiko| Kimura, Ikuro|
抄録 The inhibitory effect of calcitonin on human pancreatic secretion was evaluated to examine whether the different results reported earlier between humans, cats and dogs can be ascribed to the different sensitivity of these species to calcitonin, as suggested by some investigators. Pancreatic juice was obtained by endoscopic cannulation of the pancreatic duct from 11 patients with relapsing pancreatitis during intravenous infusion of secretin (1 U/kg/h) plus caerulein (0.04 microgram/kg/h). After steady secretion was attained 20 min after the beginning of collection, five 2-min fractions were obtained before, and ten 2-min fractions were obtained after intravenous infusion of calcitonin (1 IU/kg/h). The pre- and post-calcitonin fractions from each patient were compared by Student's t-test. Calcitonin inhibited the secretory volume (26.8 to 65.6%) and bicarbonate secretion (21.4 to 62.0%) in 8 patients, and amylase (48.4 to 89.5%) and lipase secretion (47.4 to 90.5%) in all patients. The present studies reconfirmed that prominent inhibition of enzyme secretion occurs in humans. A new finding was that significant inhibition of the secretory volume and bicarbonate secretion occurs in humans. The inhibitory effects of calcitonin in humans did not appear to differ from those in cats and dogs, when evaluated similarly with the use of pure pancreatic juice.
キーワード human pancreatic secretion calcitonin pure pancreatic juice
Amo Type Article
発行日 1989-06
出版物タイトル Acta Medica Okayama
43巻
3号
出版者 Okayama University Medical School
開始ページ 169
終了ページ 174
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 2763866
Web of Sience KeyUT A1989AG01600005
JaLCDOI 10.18926/AMO/32675
フルテキストURL fulltext.pdf
著者 Tanizaki, Yoshiro| Kitani, Hikaru| Okazaki, Morihiro| Mifune, Takashi| Mitsunobu, Fumihiro| Ochi, Koji| Harada, Hideo| Kimura, Ikuro|
抄録 The improvement of ventilatory function by spa therapy was examined in 37 patients with steroid-dependent intractable asthma (SDIA) in relation to clinical asthma types. All subjects had been on long-term corticosteroid therapy before spa therapy. Spa therapy was found to improve the values of ventilatory parameters. The percent improvement in all subjects was +4.1% in %FVC, +8.9% in FEV1.0%, +9.8% in %PEFR, +22.0% in %MMF, +19.7% in %V50 and +28.2% in %V25, respectively. Relating to clinical asthma types, moderate improvement in %MMF and %V25 from 16.7% to 16.9% was observed in type Ia cases, and marked increase in %MMF, %V50 and %V25 was observed from 42.2% to 43.2% in type Ib cases. However, no significant increase was found in these parameters of types Ia or Ib after spa therapy. In patients with type II, a significant increase was shown in %V50 (p less than 0.05) and %V25 (p less than 0.01) after spa therapy. The results show that spa therapy improves the condition of small airways disorder in patients with SDIA.
キーワード spa therapy bronchial asthma ventilatory function small airways
Amo Type Article
発行日 1992-06
出版物タイトル Acta Medica Okayama
46巻
3号
出版者 Okayama University Medical School
開始ページ 175
終了ページ 178
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 1502922
Web of Sience KeyUT A1992JB50400005
JaLCDOI 10.18926/AMO/32622
フルテキストURL fulltext.pdf
著者 Tanizaki, Yoshiro| Kitani, Hikaru| Okazaki, Morihiro| Mifune, Takashi| Mitsunobu, Fumihiro| Tanimizu, Masakuni| Honke, Naoko| Kusaura, Yasuharu| Ochi, Koji| Harada, Hideo| Soda, Ryo| Takahashi, Kiyoshi| Kimura, Ikuro|
抄録 Twenty-one patients with atopic asthma were classified into three types according to their symptoms (clinical diagnosis): Ia, simple bronchoconstriction; Ib, bronchoconstriction + hypersecretion; and II, bronchiolar obstruction, and this classification was compared with a classification made according to clinical findings and examinations (score diagnosis). Type Ib asthma was characterized by the increased incidence of eosinophils in bronchoalveolar lavage fluid (BALF), while type II was characterized by ventilatory dysfunction in small airways and the increased incidence of neutrophils in BALF. Four patients, whose expectoration was between 50 and 99ml/day, of the 12 with type Ia assessed by clinical diagnosis were evaluated as type Ib by score diagnosis. One patient with type II by clinical diagnosis was assessed as questionable type II by score diagnosis. In the other 16 patients, the clinical and score diagnoses were the same.
キーワード bronchial asthma classification ventilatory function cellular composition of BALF sore diagnosis
Amo Type Article
発行日 1992-08
出版物タイトル Acta Medica Okayama
46巻
4号
出版者 Okayama University Medical School
開始ページ 295
終了ページ 301
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 1442153
Web of Sience KeyUT A1992JL44200010
JaLCDOI 10.18926/AMO/31341
フルテキストURL fulltext.pdf
著者 Mizushima, Takaaki| Ochi, Koji| Seno, Toshinobu| Matsumura, Naoki| Harada, Hideo|
抄録 We simultaneously assessed gastric emptying and gallbladder contraction after oral administration of a liquid meal by noninvasive ultrasonography in 17 patients with chronic pancreatitis (CP) and in 17 healthy controls. Gastrointestinal (GI) transit was also assessed by a noninvasive radioopaque marker method. Exocrine pancreatic function was evaluated by analyzing pure pancreatic juice and by analyzing the autonomic nervous system by cardiovascular reflex tests. Patients with CP showed impaired gallbladder contraction at 15 min and hastened gastric emptying. The cause of the former is unclear, whereas the latter was closely related with decreased pancreatic lipase output, but not with autonomic dysfunction. GI transit time did not differ between controls and patients with CP. In conclusion, we succeeded in clearly demonstrating impaired gallbladder contraction and hastened gastric emptying in patients with CP by a single noninvasive test, ultrasonography. We also revealed for the first time that hastened gastric emptying is associated with insufficient pancreatic lipase output.
キーワード chronic pancreatits gastric emptying gallbladder motility colonic transit lipase output
Amo Type Article
発行日 1998-02
出版物タイトル Acta Medica Okayama
52巻
1号
出版者 Okayama University Medical School
開始ページ 55
終了ページ 61
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 9548995
Web of Sience KeyUT 000072264100008
JaLCDOI 10.18926/AMO/31682
フルテキストURL fulltext.pdf
著者 Shinji, Toshiyuki| Ujike, Kozo| Ochi, Koji| Kusano, Nobuchika| Kikui, Tetsuya| Matsumura, Naoki| Emori, Yasuyuki| Seno, Toshinobu| Koide, Norio|
抄録 In studies of the pathogenesis of pancreatic fibrosis, pancreatic stellate cells (PSCs) have recently gained attention. In the present study, we established a new collagenase perfusion method through thoracic aorta cannulation to isolate PSCs, and we studied gene expression of TGF-beta1, type I collagen, and connective tissue growth factor using primary cultured PSCs. Our method facilitated PSC isolation, and by our new method, 4.3 +/- 1.2 x 10(6) PSCs were obtained from a rat. In comparing the expression of these genes with that of hepatic stellate cells (HSCs), we observed a similar pattern, although PSCs expressed type I collagen gene earlier than did HSCs. These results suggest that PSCs may play an important role in fibrosis of the pancreas, as HSCs do in liver fibrosis; in addition, PSCs may exist in a preactivated state or may be more easily activated than are HSCs. We also isolated the PSCs from a WBN/Kob rat, the spontaneous pancreatitis rat, and compared the gene expression with that from a normal rat.
キーワード pancreatic stellate cell transforming growth factor beta connective tissue growth factor collagenase perfusion WBN/Kob rat
Amo Type Article
発行日 2002-08
出版物タイトル Acta Medica Okayama
56巻
4号
出版者 Okayama University Medical School
開始ページ 211
終了ページ 218
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 Copyright© 2002 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 12199527
Web of Sience KeyUT 000177382600007
JaLCDOI 10.18926/AMO/32110
フルテキストURL fulltext.pdf
著者 Shinji, Toshiyuki| Kyaw, Yi Yi| Gokan, Katsunori| Tanaka, Yasuhito| Ochi, Koji| Kusano, Nobuchika| Mizushima, Takaaki| Fujioka, Shin-ichi| Shiraha, Hidenori| Lwin, Aye Aye| Shiratori, Yasushi| Mizokami, Masashi| Khin, Myo| Miyahara, Masayuki| Okada, Shigeru| Koide, Norio|
抄録 The prevalence of hepatitis C virus (HCV) genotypes in Myanmar in comparison with the rest of Southeast Asia is not well known. Serum samples were obtained from 201 HCV antibody-positive volunteer blood donors in and around the Myanmar city of Yangon. Of these, the antibody titers of 101 samples were checked by serial dilution using HCV antibody PA test II and Terasaki microplate as a low-cost method. To compare antibody titers by this method and RNA identification, we also checked HCV-RNA using the Amplicor 2.0 test. Most high-titer groups were positive for HCV-RNA. Of the 201 samples, 110 were successfully polymerase chain reaction (PCR) amplified. Among them, 35 (31.8%) were of genotype 1, 52 (47.3%) were of genotype 3, and 23 (20.9%) were of type 6 variants, and phylogenetic analysis of these type 6 variants revealed that 3 new type 6 subgroups exist in Myanmar. We named the subgroups M6-1, M6-2, and M6-3. M6-1 and M6-2 were relatively close to types 8 and 9, respectively. M6-3, though only found in one sample, was a brand-new subgroup. These subtypes were not seen in Vietnam, where type 6 group variants are widely spread. These findings may be useful for analyzing how and when these subgroups were formed.
キーワード hepatitis C virus(HCV)genotype type 6 variant Myanmar Southeast Asia phylogenetic analysis
Amo Type Article
発行日 2004-06
出版物タイトル Acta Medica Okayama
58巻
3号
出版者 Okayama University Medical School
開始ページ 135
終了ページ 142
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 15471435
Web of Sience KeyUT 000222273300004
JaLCDOI 10.18926/AMO/48563
フルテキストURL 66_3_239.pdf
著者 Sawa, Kiminari| Mizushima, Takaaki| Matsushita, Koki| Shirahige, Akinori| Ochi, Koji| Koide, Norio|
抄録 Plain abdominal radiography is a very basic examination and plays an important role in primary care. The objectives of this study were to clarify colon distributions on plain abdominal radiographs. Forty-three healthy volunteers underwent gastric fluoroscopy, and 2 hours later, plain abdominal radiography in the supine position. A region of interest (ROI) was defined uniformly on each X-ray image to divide the image into 600 zones. The area corresponding to the large bowel within the ROI was divided into 4 segments (ascending colon, transverse colon, descending colon, and sigmoid colon+rectum). The percentage of barium in each segment relative to the total volume of barium used was calculated to evaluate the percent ROI occupancy. The large bowel covered 76.7% of the entire ROI, with the percent duplication being 55%. The duplicated area corresponded to the transverse colon region. When the method proposed by Arhan et al. was used, the percentage of the colon actually present in each segment relative to that determined theoretically was 99.6% for the right colon segment, 92.2% for the left colon segment, and 92.2% for the sigmoid/rectal segment. However, in cases in which the transverse colon descended partially from the fifth lumbar vertebra, the percentage occupied by the sigmoid colon+rectum decreased to 57.2%. We applied a new large bowel segmentation method especially for patients with ptosis, by devising a line joining the lateral side of the right lesser pelvis and the lower ends of both sacroiliac joints.
キーワード large bowel segmentation plain abdominal radiograph classification method primary care
Amo Type Original Article
発行日 2012-06
出版物タイトル Acta Medica Okayama
66巻
3号
出版者 Okayama University Medical School
開始ページ 239
終了ページ 244
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2012 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 22729104
Web of Sience KeyUT 000305669700007
関連URL http://ousar.lib.okayama-u.ac.jp/metadata/48881
JaLCDOI 10.18926/AMO/48687
フルテキストURL 66_4_329.pdf
著者 Matsushita, Koki| Mizushima, Takaaki| Shirahige, Akinori| Tanioka, Hiroaki| Sawa, Kiminari| Ochi, Koji| Tanimoto, Mitsune| Koide, Norio|
抄録 The relationship between pancreatic fibrosis and apoptosis of pancreatic acinar cells has not been fully elucidated. We reported that taurine had an anti-fibrotic effect in a dibutyltin dichloride (DBTC)-chronic pancreatitis model. However, the effect of taurine on apoptosis of pancreatic acinar cells is still unclear. Therefore, we examined apoptosis in DBTC-chronic pancreatitis and in the AR42J pancreatic acinar cell line with/without taurine. Pancreatic fibrosis was induced by a single administration of DBTC. Rats were fed a taurine-containing diet or a normal diet and were sacrificed at day 5. The AR42J pancreatic acinar cell line was incubated with/without DBTC with taurine chloramines. Apoptosis was determined by using terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling (TUNEL) assay. The expression of Bad and Bcl-2 proteins in the AR42J cells lysates was detected by Western blot analysis. The apoptotic index of pancreatic acinar cells in DBTC-administered rats was significantly increased. Taurine treatment inhibited pancreatic fibrosis and apoptosis of acinar cells induced by DBTC. The number of TUNEL-positive cells in the AR42J pancreatic acinar cell lines was significantly increased by the addition of DBTC. Incubation with taurine chloramines ameliorated these changes. In conclusion, taurine inhibits apoptosis of pancreatic acinar cells and pancreatitis in experimental chronic pancreatitis.
キーワード apoptosis chronic pancreatitis pancreatic acinar cells taurine
Amo Type Original Article
発行日 2012-08
出版物タイトル Acta Medica Okayama
66巻
4号
出版者 Okayama University Medical School
開始ページ 329
終了ページ 334
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2012 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 22918205
Web of Sience KeyUT 000307918900005
関連URL http://ousar.lib.okayama-u.ac.jp/metadata/49739