このエントリーをはてなブックマークに追加
ID 13475
Eprint ID
13475
フルテキストURL
Thumnail 117_219.pdf 5.37 MB
タイトル(別表記)
Characterization and usefulness of a monoclonal antibody against human herpesvirus-7 DNA polymerase processivity factor
著者
難波 ひかる 岡山大学大学院医歯薬学総合研究科 病原ウイルス学 Kaken ID researchmap
抄録
A monoclonal antibody (MAb), IK17, was developed and found useful for the analysis of human herpesvirus-7 (HHV7) genome replication. In Western blot analysis, the MAb IK17 specifically detected an HHV7 DNA polymerase subunit,U27 protein,expressed in both Escherichia coli (E.Coli)and HHV7-infected cells.Analysis by the same method of truncated U27 proteins expressed in E. Coli demonstrated that the MAb IK17 recognizes an epitope between amino acid 1 and 133 of the U27 protein.The indirect immunofluorescence (IF)method with MAb IK17 detected an unexpected reduction of U27 protein in the infected cells that were treated with a viral DNA polymerase inhibitor,phosphonoacetic acid (PAA),although no reduction of U27 mRNA was observed in the cells by Northern blot analysis. The data suggest the possible usefulness of the MAb IK17 for obtaining clues to characterize an unknown feature of the U27 protein. Immunoprecipitation experiments with the MAb IK17 enabled to detect at least four U27 protein-associated phosphorylated polypeptides in addition to the U27 protein itself.The result indicates the usefulness of the MAb IK17 for qualitative and quantitative analysis of the U27 proteinassociated viral and cellular factors. These analyses contribute to elucidation of the HHV7 genome replication system.
キーワード
ヒトヘルペスウイルス7型 (Human Herpesvirus-7)
DNA polymerase processivity factor
単クローン抗体 (monoclonal antibody)
備考
原著
発行日
2006-01-04
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
117巻
3号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
219
終了ページ
224
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/117/3/117_3_219/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/
言語
Japanese
著作権者
Copyright© 岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma