著者 Yasuda, Yukiko| Sakai, Akiko| Ito, Sachio| Sasai, Kaori| Yamamoto, Hiromasa| Matsubara, Nagahide| Ouchida, Mamoru| Katayama, Hiroshi| Shimizu, Kenji|
発行日 2017-02
出版物タイトル Acta Medica Okayama
71巻
1号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/54826
著者 Maki, Yu| Murakami, Jun| Asaumi, Jun-ichi| Tsujigiwa, Hidetsugu| Nagatsuka, Hitoshi| Kokeguchi, Susumu| Fukui, Kazuhiro| Kawai, Noriko| Yanagi, Yoshinobu| Kuroda, Masahiro| Tanaka, Noriaki| Matsubara, Nagahide| Kishi, Kanji|
発行日 2005-7
出版物タイトル Oral Oncology
41巻
10号
資料タイプ 学術雑誌論文
著者 Murakami, Jun| Asaumi, Jun-ichi| Kawai, Noriko| Tsujigiwa, Hidetsugu| Yanagi, Yoshinobu| Nagatsuka, Hitoshi| Inoue, Tetsuyoshi| Kokeguchi, Susumu| Kawasaki, Shoji| Kuroda, Masahiro| Tanaka, Noriaki| Matsubara, Nagahide| Kishi, Kanji|
発行日 2005-07
出版物タイトル Cancer Chemotherapy and Pharmacology
56巻
1号
資料タイプ 学術雑誌論文
著者 Murakami, Jun| Asaumi, Jun-ichi| Maki, Yuu| Tsujigiwa, Hidetsugu| Kuroda, Masahiro| Nagai, Noriyuki| Yanagi, Yoshinobu| Inoue, Tetsuyoshi| Kawasaki, Shoji| Tanaka, Noriaki| Matsubara, Nagahide| Kishi, Kanji|
発行日 2004-2
出版物タイトル Oral Oncology
40巻
6号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/32201
フルテキストURL fulltext.pdf
著者 Shiiki, Sigeo| Fuchimoto, Sadanori| Iwagaki, Hiromi| Akazai, Yoshihiro| Matsubara, Nagahide| Watanabe, Tetsuya| Orita, Kunzo|
抄録 <p>We investigated the antitumor activities of 5-fluorouracil (5-FU), 5'-deoxy-5-fluorouridine (5'-DFUR), 1-hexylcarbamoyl-5-fluorouracil (HCFU) and 1-(tetrahydro-2-furanyl)-5-fluorouracil (FT-207) in combination with hyperthermia in vitro. The antitumor effect of 5-FU (10(-4) M) was slightly enhanced by combination with hyperthermia (42 degrees C) for 2h, and the effect was determined to be additive. Synergistic enhancement of antitumor activity was obtained by the concurrent use of hyperthermia (42 degrees C, 2h) and 5'-DFUR (10(-4) M) or HCFU (10(-5) M). However, the antitumor effect of FT-207 (10(-4) M) in combination with hyperthermia was comparable that of hyperthermia alone. The synergistic enhancement of antitumor activity was not obtained for all drugs when the cells were preheated at 42 degrees C for 2h. On the other hand, when cells were pretreated with drugs before heat exposure, weak interactions were obtained after 5-FU and 5'-DFUR treatment, and a synergistic interaction was obtained after HCFU treatment. It is speculated that the metabolites of 5'-DFUR and HCFU enhance the cytotoxicity of 5-FU, or might change the threshold concentration for a cytotoxic effect of 5-FU in cancer cells.</p>
キーワード hyperthermia 5-fluorouridine 5'-deoxy-5-fluorouridine 1-hexylcarbomoyl-5-fluorouracil FT-207
Amo Type Article
発行日 1991-10
出版物タイトル Acta Medica Okayama
45巻
5号
出版者 Okayama University Medical School
開始ページ 339
終了ページ 345
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 1836706
Web of Sience KeyUT A1991GN53800008
JaLCDOI 10.18926/AMO/30741
フルテキストURL fulltext.pdf
著者 Nagasaka, Takeshi| Goel, Ajay| Matsubara, Nagahide| Tanaka, Noriaki|
抄録 Aberrant promoter methylation, an 'epigenetic' form of genomic instability that leads to transcriptional silencing of tumor suppressor genes, is increasingly being recognized as a crucial component in the evolution of human cancers. With our limited knowledge of the molecular basis and timing of the initiation of altered methylation events in the stepwise progression of cancers, the biggest challenge we currently face is to identify novel biomarkers and technologies for the timely screening of patients carrying such alterations. One such strategy would be to develop tests for the detection of fecal DNA methylation patterns that will improve the sensitivity of noninvasive screening tests for colorectal neoplasia, and moreover, will decrease both mortality and the incremental costs of treating colorectal cancers.
キーワード fecal DNA colorectal cancer methylation epigenetics
Amo Type Review
発行日 2006-10
出版物タイトル Acta Medica Okayama
60巻
5号
出版者 Okayama University Medical School
開始ページ 249
終了ページ 256
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 17072371
Web of Science KeyUT 000241509000001
JaLCDOI 10.18926/AMO/30407
フルテキストURL fulltext.pdf
著者 Hamazaki, Keisuke| Okamoto, Ko| Gochi, Akira| Matsubara, Nagahide| Mori, Masanobu| Orita, Kunzo|
抄録 <p>A persistent problem in orthotopic liver transplantation is primary nonfunction (PNF) of the hepatic allograft. In an attempt to reduce the incidence of graft failure, the feasibility of pretransplant assessment of graft viability was investigated by 31P nuclear magnetic resonance (NMR) spectroscopy. The level of adenosine triphosphate (ATP) was measured as an indicator of liver function by 31P NMR spectroscopy after a 30 min normothermic reperfusion following cold-storage in University of Wisconsin (UW) solution. The mean +/- SD beta-ATP/Pi ratio after preservation for 0, 12, 24 or 48 h was 1.40 +/- 0.34, 0.85 +/- 0.27, 0.64 +/- 0.14 and 0.38 +/- 0.09, respectively. Significance was observed between 12h and 24h and between 12h and 48h of preservation. These results correlated well with the morphological changes in endothelial cells and sinusoidal lining cells examined by transmission electron microscopy. It is suggested strongly that microcirculatory disturbances due to endothelial cell injury impairs the recovery of ATP levels after reperfusion, and that ATP determination by 31P NMR spectroscopy, as a non-invasive modality, may help in the prediction of PNF after liver transplantation.</p>
キーワード 31P-NMR liver preservation UW solution
Amo Type Article
発行日 1995-06
出版物タイトル Acta Medica Okayama
49巻
3号
出版者 Okayama University Medical School
開始ページ 175
終了ページ 178
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 7676849
Web of Sience KeyUT A1995RH05400009
JaLCDOI 10.18926/AMO/30382
フルテキストURL fulltext.pdf
著者 Iwagaki, Hiromi| Hamazaki, Keisuke| Matsubara, Nagahide| Hiramatsu, Midori| Orita, Kunzo| Mori, Akitane|
抄録 <p>In this study, we measured free radicals and thiobarbituric acid-reactive substances (TBARS) in hepatocellular carcinoma and in non-cancerous liver parenchyma. There was a higher concentration of free radicals in malignant tissue than in non-cancerous tissue. In contrast, the level of TBARS was significantly (P &#60; 0.01) lower than non-cancerous liver parenchyma. These paradoxical results suggested that antioxidative enzyme activity and/or inhibition of lipid peroxidation were higher in hepatocellular carcinoma.</p>
キーワード hepatocellular carcinoma free radicals lipid peroxidation
Amo Type Article
発行日 1995-12
出版物タイトル Acta Medica Okayama
49巻
6号
出版者 Okayama University Medical School
開始ページ 313
終了ページ 315
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8770241
Web of Science KeyUT A1995TM84600007
JaLCDOI 10.18926/AMO/30379
フルテキストURL fulltext.pdf
著者 Hamazaki, Keisuke| Gochi, Akira| Matsubara, Nagahide| Mori, Mazanobu| Orita, Kunzo|
抄録 <p>Fas antigen (ag) is a cell surface protein known to trigger apoptosis in a variety of cells upon specific antibody binding. On the other hand, Bcl-2 protein, an oncogene product located at the mitochondrial inner surface, prolongs cell survival by blocking apoptosis. In this study we examined the expression of Fas ag and bcl-2 protein in 17 cases of hepatocellular carcinoma (HCC) to determine their role on HCC. By flow cytometric analysis, mean (SD) value of the expression of Fas ag on hepatocytes derived from normal liver, diseased liver (chronic hepatitis or liver cirrhosis) and HCC was 5.8 (4.7)%, 10.3 (6.9)%, and 24.0 (18.2)%, respectively. Fas ag expression on hepatoma cells was significantly greater than normal and diseased liver cells. The expression of Bcl-2 protein in normal liver, diseased liver and HCC was 4.3 (8.5)%, 0.8 (2.5)% and 2.1 (3.4)%, respectively, and the difference was not significant. These results suggest that induction of apoptosis may be a possible therapy against HCC.</p>
キーワード apoptosis Fas antigen Bcl-2 hepatocellular carcinoma
Amo Type Article
発行日 1995-08
出版物タイトル Acta Medica Okayama
49巻
4号
出版者 Okayama University Medical School
開始ページ 227
終了ページ 230
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 7502684
Web of Science KeyUT A1995RR97800008