JaLCDOI 10.18926/AMO/31744
フルテキストURL fulltext.pdf
著者 Hayashi, Hisatomo| Tsuda, Takashi| Tsurumi, Naokazu| Takai, Yutaka| Maeda, Masanori| Takahashi, Kiyoshi| Kimura, Ikuro|
抄録 <p>A significant amount of anticoagulant substance was released along with histamine, when human lung mast cells were stimulated with anti-IgE and Ca-ionophore A23187. Its activity was lost by heparinase, not by chondroitin-ABC lyase or chondroitin-AC lyase, and also inhibited by Polybrene, suggesting it would be heparin.</p>
キーワード human lung mast cells heparin anticoagulant activity anti-IgE Ca-ionophore
Amo Type Article
発行日 1987-04
出版物タイトル Acta Medica Okayama
41巻
2号
出版者 Okayama University Medical School
開始ページ 85
終了ページ 87
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 2438904
Web of Sience KeyUT A1987H040200006
JaLCDOI 10.18926/AMO/31572
フルテキストURL fulltext.pdf
著者 Tanabe, Kozo| Takahashi, Kiyoshi| Maeda, Masanori| Kimura, Ikuro|
抄録 <p>To confirm the formation of Charcot-Leyden crystals (CLC) in basophils, we observed basophils in sputum and peripheral blood. Sealed slide and suspension culture methods were used to observe the process of CLC formation in peripheral blood basophils and eosinophils under electron microscopy. CLC formation was observed in basophils and eosinophils, and was found to be augmented by sealed slide method. A temperature of 4 degrees C was better than 37 degrees C for promoting the formation of crystals. There was no correlation between the degranulation of these cells and the formation of CLC after stimulation with anti-IgE or anti-IgG antibodies. CLC were initially detected in the cytoplasmic granules of basophils where they continued to enlarge. No CLC were identified in mast cells under any conditions studied. These findings confirm that CLC in sputum are not exclusive to eosinophils and that CLC appear to be present in basophil-rich sites under the cell damage.</p>
キーワード Charcot-Leyden crystals basphils electron microscopy
Amo Type Article
発行日 1993-04
出版物タイトル Acta Medica Okayama
47巻
2号
出版者 Okayama University Medical School
開始ページ 85
終了ページ 90
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8506754
Web of Sience KeyUT A1993LA45200003
JaLCDOI 10.18926/AMO/31498
フルテキストURL fulltext.pdf
著者 Tanizaki, Yoshiro| Komagoe, Haruki| Morinaga, Hiroshi| Ohtani, Jun| Nakagawa, Saburo| Maeda, Masanori| Kitani, Hikaru| Takahashi, Kiyoshi| Kimura, Ikuro|
抄録 <p>The inhibitory effect of nicardipine, a calcium antagonist, on the antigen- and anti-IgE-induced histamine release from basophilic leucocytes of patients with bronchial asthma was examined. The agent significantly inhibited both antigen-stimulated and anti-IgE-induced histamine release from basophils (the maximum percent inhibition was 57.8 +/- 7.2% and 56.0 +/- 8.8%, respectively). Pre-incubation of basophils with nicardipine for periods of up to 120 min did not alter the inhibitory effect. These results suggest that nicardipine modifies the histamine release from basophils which closely participate in an attack of bronchial asthma.</p>
キーワード histamine release basophils antigen anti-IgE Ca<sup>2+</sup> antagonist
Amo Type Article
発行日 1985-08
出版物タイトル Acta Medica Okayama
39巻
4号
出版者 Okayama University Medical School
開始ページ 247
終了ページ 251
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 2413722
Web of Sience KeyUT A1985APN0700001