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ID 52818
フルテキストURL
著者
Iida, Sachiyo Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Occlusal & Oral Funct Rehabil
Hara, Tetsuya Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Occlusal & Oral Funct Rehabil
Araki, Daisuke Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Occlusal & Oral Funct Rehabil
Ishimine-Kuroda, Chisa Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Occlusal & Oral Funct Rehabil
Kurozumi, Akimasa Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Occlusal & Oral Funct Rehabil
Sakamoto, Shunichi Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Occlusal & Oral Funct Rehabil
Miyazaki, Takako Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Occlusal & Oral Funct Rehabil
Minagi, Shogo Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Occlusal & Oral Funct Rehabil
抄録
Objectives: The present study aimed to identify the effect of memory-related genes on male rats tested for spatial memory with either molar teeth extraction or its restoration by occlusal support using experimental dentures. Design: Memory-related genes were detected from hippocampi of male Wistar rats (exposed to teeth extraction with or without dentures, or no extraction (control)) (7-week old) after behavioural testing (via the radial maze task) using a DNA microarray. The time course of the expression of these genes was evaluated by quantitative real-time polymerase chain reaction (PCR) (on 49-week-old rats). Results: In preliminary experiments, to determine which memory genes are affected by spatial memory training, DNA microarray analysis revealed that thyrotropin-releasing hormone (Trh) and tenascin XA (Tnxa) were up-regulated and neuronatin (Nnat) and S100a9 were down-regulated after the maze training. The expression of Tnxa, Nnat and S100a9 of 49-week-old rats (during the time course) via quantitative real-time PCR was consistent with the results of microarrays of the preliminary experiment. Expression of Trh that was evaluated by quantitative real-time PCR did not agree with the results for this gene from the microarray for all groups. Therefore, expression of Trh may have increased in only young, trained rats. The expression of S100a9 prior to the maze task was down-regulated in only the extraction group. Conclusion: These results demonstrated that Trh, Tnxa and Nnat genes were affected according to the degree of memory in male rats. This study also indicated that S100a9 is a memory-related gene, which is affected by the presence of occlusal support.
キーワード
Gene expression
Memory and learning
Hippocampus
Trh
Tnxa
Nnat
S100a9
発行日
2014-02
出版物タイトル
Archives of Oral Biology
59巻
2号
出版者
Pergamon-Elsevier Science Ltd.
開始ページ
133
終了ページ
141
ISSN
0003-9969
NCID
AA00548457
資料タイプ
学術雑誌論文
関連URL
http://ousar.lib.okayama-u.ac.jp/metadata/52555
言語
English
著作権者
© 2013 Elsevier Ltd. All rights reserved.
論文のバージョン
author
査読
有り
DOI
Web of Sience KeyUT