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ID 54548
フルテキストURL
著者
大槻 高史 岡山大学大学院自然科学研究科
Kanzaki, Shigeto Department of Biomedical Engineering, Okayama University
Nishimura, Sae Department of Biomedical Engineering, Okayama University
Kunihiro, Yoshio Department of Biomedical Engineering, Okayama University
Sisido, Masahiko Department of Biomedical Engineering, Okayama University
渡邉 和則 岡山大学大学院自然科学研究科
抄録
The possibility of spatiotemporally photocontrolling translation holds considerable promise for studies on the biological roles of local translation in cells and tissues. Here we report caged aminoacyl-tRNAs (aa-tRNAs) synthesized using a (7-diethylaminocoumarin-4-yl)methoxycarbonyl (DEACM)-cage compound. DEACM-caged aa-tRNA does not spontaneously deacylate for at least 4 h in neutral aqueous solution, and does not bind to the elongation factor Tu. On irradiation at ∼405 nm at 125 mW cm(-2), DEACM-aa-tRNA is converted into active aa-tRNA with a half-life of 19 s. Notably, this rapid uncaging induced by visible light does not impair the translation system. Translation is photoinduced when DEACM-aa-tRNA carrying a CCCG or a CUA anticodon is uncaged in the presence of mRNAs harbouring a CGGG four-base codon or a UAG amber codon, respectively. Protein synthesis is phototriggered in several model systems, including an in vitro translation system, an agarose gel, in liposomes and in mammalian cells.
備考
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発行日
2016-08
出版物タイトル
Nature Communications
7巻
出版者(別表記)
Macmillan Publishers Limited, part of Springer Nature
開始ページ
12501
終了ページ
12501
ISSN
2041-1723
NCID
AA12645905
資料タイプ
学術雑誌論文
オフィシャル URL
http://www.nature.com/ncomms/
言語
English
著作権者
© The Author(s) 2016
論文のバージョン
publisher
査読
有り
DOI
PubMed ID
Web of Sience KeyUT