著者 座間味 義人| 高取 真吾| 合田 光寛| 小山 敏広| 岩谷 有希子| Jin, Xin| Takada-Doi, Shima| 川﨑 博己|
発行日 2008-11-01
出版物タイトル Biological & Pharmaceutical Bulletin
31巻
11号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/30965
フルテキストURL fulltext.pdf
著者 Jin, Xin| Otonashi-Satoh, Yukiko| Sun, Pengyuan| Kawamura, Naomi| Tsuboi, Takashi| Yamaguchi, Yasuyo| Ueda, Taro| Kawasaki, Hiromu|
抄録 <p>The vascular effects of an aqueous extract prepared from the leaves of Eucommia ulmoides Oliv. (ELE), a medicinal herb commonly used in antihypertensive herbal prescriptions in China, were investigated in rat mesenteric resistance arteries. The mesenteric vascular bed was perfused with Krebs solution and the perfusion pressure was measured with a pressure transducer. In preparations with an intact endothelium and precontracted with 7&#956;M methoxamine, perfusion of ELE (10<sup>&#65293;7</sup>&#65293;10<sup>&#65293;2</sup>mg/ml for 15min) caused a concentration-dependent vasodilatation, which was abolished by chemical removal of the endothelium. The ELE-induced vasodilatation was inhibited by neither indomethacin (INDO, a cyclooxygenase inhibitor) nor N<sup>G</sup>-nitro-L-arginine-methyl ester (L-NAME, a nitric oxide inhibitor). The ELE-induced vasodilatation was significantly inhibited by tetraethylammonium (TEA, a K<sup>+</sup> channel blocker) and 18&#945;-glycyrrhetinic acid (18&#945;-GA, a gap-junction inhibitor), and abolished by high K<sup>+</sup> -containing Krebs&#700; solution. Atropine (a muscarinic acetylcholine receptor antagonist) significantly inhibited the vasodilatation induced by ELE at high concentrations. These results suggest that the ELE-induced vasodilatation is endothelium-dependent but nitric oxide (NO)- and prostaglandin I<sub>2</sub> (PGI<sub>2</sub>)-independent, and is mainly mediated by the endothelium-derived hyperpolarizing factor (EDHF) in the mesenteric resistance arteries. Furthermore, the ELE-induced EDHF-mediated response involves the activation of K<sup>+</sup>-channels and gap junctions.</p>
キーワード Eucommia ulmoides Oliv. leaf extract endothelium-dependent vasodilation endothelium-derived hyperpolarizing factor mesenteric resistance artery
Amo Type Original Article
発行日 2008-10
出版物タイトル Acta Medica Okayama
62巻
5号
出版者 Okayama University Medical School
開始ページ 319
終了ページ 325
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 18985092
Web of Sience KeyUT 000260391300006