著者 Sing’ombe Ombiro Geofrey| Sawai, Taku| Noutoshi, Yoshiteru| Nishina, Yuta| Matsui, Hidenori| Yamamoto, Mikihiro| Toyoda, Kazuhiro| Ichinose, Yuki|
抄録 Plant pathogenic bacteria cause huge yield losses in crops globally. Therefore, finding effective bactericides to these pathogens is an immediate challenge. In this study, we sought compounds that specifically inhibit the growth of Ralstonia solanacearum. As a result, we identified one promising compound, 1-(4-bromophenyl)-6-methoxy-2,3,4,9-tetrahydro-1H-β-carboline, which inhibited the growth of R. solanacearum (Rs1002) from a pilot library of 376 chemicals provided from RIKEN. We further obtained its structural analogues and assessed their ability to inhibit Rs1002 growth. Then we identified five compounds, named ralhibitins A to E, that specifically inhibit growth of Rs1002 at >5 μg/ml final concentration. The most effective compounds, ralhibitins A, C, and E completely inhibited the growth of Rs1002 at 1.25 μg/ml. In addition, ralhibitins A to E inhibited growth of Xanthomonas oryzae pv. oryzae but not the other bacteria tested at a final concentration of 10 μg/ml. Whereas, ralhibitin E, besides inhibiting R. solanacearum and X. oryzae pv. oryzae, completely inhibited the growth of X. campestris pv. campestris and the Gram-positive bacterium Clavibacter michiganensis subsp. michiganensis at 10 μg/ml. Growth inhibition by these compounds was stable at pH 6–9 and after autoclaving. Because Rs1002 grew in the culture medium in which ralhibitins were incubated with the ralhibitin-insensitive bacteria, the unaffected bacteria may be able to inactivate the inhibitory effect of ralhibitins. These results suggest that ralhibitins might be potential lead compounds for the specific control of phytopathogenic bacteria.
キーワード Bactericide Ralhibitins Ralstonia solanacearum Lead compounds
備考 This is an Accepted Manuscript of an article published by Elsevier This fulltext will be available in June 2019
発行日 2018-10
出版物タイトル Microbiological Research
215巻
出版者 Elsevier
開始ページ 29
終了ページ 35
ISSN 0944-5013
NCID AA11017780
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン author
DOI 10.1016/j.micres.2018.06.005
関連URL isVersionOf https://doi.org/10.1016/j.micres.2018.06.005
フルテキストURL MolGenetGenomics20180516.pdf
著者 Sawada, Takahiro| Eguchi, Miho| Asaki, Seiya| Kashiwagi, Ryota| Shimomura, Kousuke| Taguchi, Fumiko| Matsui, Hidenori| Yamamoto, Mikihiro| Noutoshi, Yoshiteru| Toyoda, Kazuhiro| Ichinose, Yuki|
キーワード Flagella motility MexEF-OprN Multidrug efflux pump transporter N-Acyl-homoserine lactone Quorum sensing
備考 This is an Accepted Manuscript of an article published by Springer Nature 2018
発行日 2018-03-16
出版物タイトル Molecular Genetics and Genomics
293巻
4号
出版者 Springer
開始ページ 1
終了ページ 11
ISSN 16174615
NCID AA11547886
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン author
PubMed ID 29549432
DOI 10.1007/s00438-018-1430-9
Web of Sience KeyUT 000437440800011
関連URL isVersionOf https://doi.org/10.1007/s00438-018-1430-9