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ID 30686
JaLCDOI
フルテキストURL
著者
Yamada, Teruo Okayama University
Talbot, Alan Okayama University
Iijima, Yoshio Okayama University
Itano, Yoshitaro Okayama University
Kosaka, Futami Okayama University
抄録

This study used a Shimadzu IP-1B capillary type isotachophoretic apparatus with a potential gradient detector. An ipp-1 withdrawal cell was fitted to this and a technique for withdrawing individual components directly through this port was developed using a microsyringe. The recovery rate was up to 45% for individual target components. When 100% withdrawal of the target component was attempted by withdrawing a volume four times the calculated volume (so that the zones both before and after the target component were also included), the best recovery rate was only 78%. In all cases, the results varied less than 3%. The limit for analysis of individual components of a 0.01 M solution was around 3 microliters. If this volume was exceeded, the ion quantity was too large for the volume of the microcapillary tube and mixed zones formed such that complete separation and analysis of individual components became impossible.

キーワード
isotachophoresis
potential gradient detector ion mobility
ionic separation
withdrawal cell
Amo Type
Article
発行日
1982-10
出版物タイトル
Acta Medica Okayama
36巻
5号
出版者
Okayama University Medical School
開始ページ
399
終了ページ
406
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT