JaLCDOI 10.18926/AMO/31767
フルテキストURL fulltext.pdf
著者 Takahashi, Tooru| Iijima, Yoshio| Matsumi, Masaki| Abe, Shinya| Itano, Yoshitaro| Kosaka, Futami|
抄録 <p>The involvement of macrophages in the induction of metallothionein (MT) synthesis by bacterial endotoxin was studied in vitro. Rat peritoneal macrophages were incubated with endotoxin. The incubation medium from endotoxin-activated macrophages accelerated MT synthesis by human hepatic Chang cells. However, the incubation medium from non-activated macrophages did not. Endotoxin added to the culture medium of Chang cells was ineffective in inducing MT synthesis. The contents of zinc, copper and cadmium, which are primary inducers of MT, in the incubation medium of macrophages in the presence of endotoxin were not different from those in the absence of endotoxin. These results suggest that MT synthesis is induced by endotoxin-treated macrophages.</p>
キーワード metallothionein endotoxin macrophages Change liver cells
Amo Type Article
発行日 1987-02
出版物タイトル Acta Medica Okayama
41巻
1号
出版者 Okayama University Medical School
開始ページ 19
終了ページ 23
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 3494383
Web of Sience KeyUT A1987G146400003
JaLCDOI 10.18926/AMO/30686
フルテキストURL fulltext.pdf
著者 Yamada, Teruo| Talbot, Alan| Iijima, Yoshio| Itano, Yoshitaro| Kosaka, Futami|
抄録 <p>This study used a Shimadzu IP-1B capillary type isotachophoretic apparatus with a potential gradient detector. An ipp-1 withdrawal cell was fitted to this and a technique for withdrawing individual components directly through this port was developed using a microsyringe. The recovery rate was up to 45% for individual target components. When 100% withdrawal of the target component was attempted by withdrawing a volume four times the calculated volume (so that the zones both before and after the target component were also included), the best recovery rate was only 78%. In all cases, the results varied less than 3%. The limit for analysis of individual components of a 0.01 M solution was around 3 microliters. If this volume was exceeded, the ion quantity was too large for the volume of the microcapillary tube and mixed zones formed such that complete separation and analysis of individual components became impossible.</p>
キーワード isotachophoresis potential gradient detector ion mobility ionic separation withdrawal cell
Amo Type Article
発行日 1982-10
出版物タイトル Acta Medica Okayama
36巻
5号
出版者 Okayama University Medical School
開始ページ 399
終了ページ 406
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 7180573
Web of Sience KeyUT A1982PN03500008