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ID 31570
JaLCDOI
フルテキストURL
著者
Matsuoka, Junji Okayama Central Hospital
Sakagami, Kenichi Okayama University
Fujiwara, Toshiyoshi Okayama Univeristy ORCID 科研費研究者番号
Onoda, Tadashi Okayama University
Idani, Hitoshi Okayama University
Gochi, Akira Okayama University
Orita, Kunzo Okayama University
抄録

A sustained release system for interleukin-2 (IL-2), and IL-2 mini-pellet (IL-2 mp), was developed by fusing IL-2 into a needle shaped collagen. Serum concentration of IL-2 after a single subcutaneous injection of the IL-2 mp into C57BL/6 mice remained elevated longer than after an injection of aqueous IL-2. IL-2 in the serum became undetectable by 6h after a subcutaneous injection of 1 x 10(6) unit of IL-2 in phosphate-buffered saline (PBS). In contrast, after a single subcutaneous injection of IL-2 mp containing the same amount of IL-2, the concentration of IL-2 increased to its maximum at 6h after injection, then began to decrease gradually. IL-2 was detected even on the third day after a single subcutaneous injection of one IL-2 mp. Augmentation of NK activity and generation of IL-2 activated killer cells were observed in the spleen from day 1--day 3 after a single subcutaneous injection of IL-2 mp into C57BL/6 mice. This activation was not observed following a single subcutaneous injection of the same amount of IL-2 in PBS. Adoptive immunotherapy by a single subcutaneous injection of IL-2 mp followed by intravenous injections of in vitro cultured IL-2 activated killer cells showed better results in decreasing the number of metastases of Lewis lung carcinoma in C57BL/6 mice than immunotherapy using IL-2 solution.(ABSTRACT TRUNCATED AT 250 WORDS)

キーワード
IL-2
drug delivery system
immunotherapy
mouse
Amo Type
Article
発行日
1993-04
出版物タイトル
Acta Medica Okayama
47巻
2号
出版者
Okayama University Medical School
開始ページ
79
終了ページ
84
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT