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ID 30332
JaLCDOI
フルテキストURL
著者
Manabe, Yuichi Okayama University
Tsubota, Teruhiko Okayama University
Haruta, Yuro Okayama University
Kataoka, Kiyoshi Okayama University
Okazaki, Morihiro Okayama University
Haisa, Shigeru Okayama University
Nakamura, Kunihiko Okayama University
Kimura, Ikuro Okayama University
抄録

Macromomycin (MCR), an unique membrane-reactive anticancer antibiotic, was incubated with murine monoclonal anti-HLA IgG1 antibody (H-1) in the presence of carbodiimide. The resulting mixture was fractionated with a Sephadex G-200 column. The first and second fractions were shown to contain MCR-(H-1) conjugate by the elution profile, as well as by the Sarcina lutea growth inhibition assay and Ouchterlony double-diffusion method. A membrane immunofluorescence test with anti-MCR and anti-mouse IgG antibodies demonstrated specific localization of MCR-(H-1) on the surface of HLA-bearing NALL -1 cells. MCR-(H-1) inhibited the growth of HLA-lacking NS-1 cells statistically less effectively than MCR alone (p less than 0.01). On the other hand, the conjugate and free MCR equally inhibited the growth and 3H-TdR incorporation of HLA-bearing NALL -1 cells. These results indicate that the antibody-bound MCR retained both MCR and antibody activities, and thus exerted antibody-targeting MCR cytotoxicity in vitro.

キーワード
macromomycin(MCR)
anti-HLA monoclonal antibody
carbodiimide
Amo Type
Article
発行日
1984-04
出版物タイトル
Acta Medica Okayama
38巻
2号
出版者
Okayama University Medical School
開始ページ
117
終了ページ
124
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT