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ID 32911
JaLCDOI
フルテキストURL
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著者
Takemoto, Kei Kanazawa University
Ogino, Keiki Okayama University
Wang, Da-Hong Okayama University
Takigawa, Tomoko Okayama University
Kurosawa, Carmen M. Okayama University
Kambayashi, Yasuhiro Kanazawa University
Hibino, Yuri Kanazawa University
Hitomi, Yoshiaki Kanazawa University
Ichimura, Hiroshi Kanazawa University
抄録

It is well known that eosinophils are involved in tyrosine nitration. In this study, we evaluated tyrosine nitration by rat eosinophils isolated from peritoneal fl uid and constituent eosinophils in the stomach. Rat peritoneal eosinophils activated with 1 μM phorbol myristate acetate (PMA) and 50 μM NO2 ン showed immunostaining for nitrotyrosine only in smaller cells, despite the fact that eosinophils are capable of producing superoxide (O2·ン). Free tyrosine nitrating capacity after incubation with PMA and NO2 ン was 4-fold higher in eosinophils than in neutrophils. Catalase and ク- and コ -tocopherol inhibited free tyrosine nitration by reactive nitrogen species from eosinophils but not that by peroxynitrite. Superoxide dismutase augmented free tyrosine nitration by activated eosinophils and peroxynitrite. The concentration of nitric oxide released from eosinophils was relatively low (0.32 μM/106 cells/h) and did not contribute to the formation of nitrotyrosine. On the other hand, most constituent eosinophils constituent in the rat stomach stimulated by PMA and NO2 ン showed tyrosine nitration capacity. These results suggest that intact cells other than apoptotic-like eosinophils eluted in the intraperitoneal cavity could not generate reactive species responsible for nitration by a peroxidase-dependent mechanism. In contrast, normal eosinophils in the stomach were capable of nitration, suggesting that the characteristics of eosinophils in gastric mucosa are diff erent from those eluted in the peritoneal cavity.

キーワード
eosinophil peroxidase
reactive nitrogen species
nitrotyrosine
Amo Type
Article
発行日
2007-02
出版物タイトル
Acta Medica Okayama
61巻
1号
出版者
Okayama University Medical School
開始ページ
17
終了ページ
30
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT