このエントリーをはてなブックマークに追加
ID 32195
JaLCDOI
フルテキストURL
著者
Hongo, Toshiki First Department of Internal Medicine, Okayama University Medical School
Tomoda, Jun First Department of Internal Medicine, Okayama University Medical School
Mizuno, Motowo First Department of Internal Medicine, Okayama University Medical School
Maga, Toshirou First Department of Internal Medicine, Okayama University Medical School
Tsuji, Takao First Department of Internal Medicine, Okayama University Medical School
抄録
Mucus glycoprotein is one of the major components of gastric mucus which plays an important role in mucosal defensive mechanisms as a mucus-bicarbonate barrier. Analysis of the mucus glycoprotein synthesis is a useful tool for evaluating gastric mucosal defensive factors. UDP-galactosyltransferase (UDP-Gal-T) is one of the regulating enzymes for the synthesis of the mucus glycoprotein. In the present paper, we studied assay methods for UDP-Gal-T activity in rat gastric mucosa using radiolabeled UDP-galactose and two different kinds of acceptor proteins, namely ovomucoid and asialomucin, and analyzed effects of antisecretory agents on the UDP-Gal-T activity. We used crude supernatants of homogenized scrapings of the fundic part of rat stomach as an enzyme preparation and determined optimal conditions. In each acceptor, Mn2+ and the non-ionic detergent Triton X-100 were required for the enzyme activity. With each acceptor molecule, the type of glycosidic linkages of galactose was beta-type linkage. With asialomucin as an acceptor, UDP-Gal-T activities of rat gastric mucosa decreased after intraperitoneal administration of antisecretory agents, while change of the enzyme activity was not observed with ovomucoid as an acceptor.
キーワード
rat gastric mucosa
UDP-galactosyltransferase
ovomucoid
asialomucin
Amo Type
Article
発行日
1991-10
出版物タイトル
Acta Medica Okayama
45巻
5号
出版者
Okayama University Medical School
開始ページ
301
終了ページ
308
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
著作権者
Copyright© 1999 Okayama University Medical School
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT
関連URL
http://ousar.lib.okayama-u.ac.jp/metadata/6217