| JaLCDOI | 10.18926/AMO/30332 |
|---|---|
| フルテキストURL | fulltext.pdf |
| 著者 | Manabe, Yuichi| Tsubota, Teruhiko| Haruta, Yuro| Kataoka, Kiyoshi| Okazaki, Morihiro| Haisa, Shigeru| Nakamura, Kunihiko| Kimura, Ikuro| |
| 抄録 | <p>Macromomycin (MCR), an unique membrane-reactive anticancer antibiotic, was incubated with murine monoclonal anti-HLA IgG1 antibody (H-1) in the presence of carbodiimide. The resulting mixture was fractionated with a Sephadex G-200 column. The first and second fractions were shown to contain MCR-(H-1) conjugate by the elution profile, as well as by the Sarcina lutea growth inhibition assay and Ouchterlony double-diffusion method. A membrane immunofluorescence test with anti-MCR and anti-mouse IgG antibodies demonstrated specific localization of MCR-(H-1) on the surface of HLA-bearing NALL -1 cells. MCR-(H-1) inhibited the growth of HLA-lacking NS-1 cells statistically less effectively than MCR alone (p less than 0.01). On the other hand, the conjugate and free MCR equally inhibited the growth and 3H-TdR incorporation of HLA-bearing NALL -1 cells. These results indicate that the antibody-bound MCR retained both MCR and antibody activities, and thus exerted antibody-targeting MCR cytotoxicity in vitro.</p> |
| キーワード | macromomycin(MCR) anti-HLA monoclonal antibody carbodiimide |
| Amo Type | Article |
| 発行日 | 1984-04 |
| 出版物タイトル | Acta Medica Okayama |
| 巻 | 38巻 |
| 号 | 2号 |
| 出版者 | Okayama University Medical School |
| 開始ページ | 117 |
| 終了ページ | 124 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | English |
| 論文のバージョン | publisher |
| 査読 | 有り |
| PubMed ID | 6375268 |
| Web of Sience KeyUT | A1984SN81800003 |