Okayama University Medical School Acta Medica Okayama 0386-300X 38 2 1984 Production of a macromomycin (MCR)-monoclonal antibody conjugate and its biological activity. 117 124 EN Yuichi Manabe Teruhiko Tsubota Yuro Haruta Kiyoshi Kataoka Morihiro Okazaki Shigeru Haisa Kunihiko Nakamura Ikuro Kimura Article 10.18926/AMO/30332 <p>Macromomycin (MCR), an unique membrane-reactive anticancer antibiotic, was incubated with murine monoclonal anti-HLA IgG1 antibody (H-1) in the presence of carbodiimide. The resulting mixture was fractionated with a Sephadex G-200 column. The first and second fractions were shown to contain MCR-(H-1) conjugate by the elution profile, as well as by the Sarcina lutea growth inhibition assay and Ouchterlony double-diffusion method. A membrane immunofluorescence test with anti-MCR and anti-mouse IgG antibodies demonstrated specific localization of MCR-(H-1) on the surface of HLA-bearing NALL -1 cells. MCR-(H-1) inhibited the growth of HLA-lacking NS-1 cells statistically less effectively than MCR alone (p less than 0.01). On the other hand, the conjugate and free MCR equally inhibited the growth and 3H-TdR incorporation of HLA-bearing NALL -1 cells. These results indicate that the antibody-bound MCR retained both MCR and antibody activities, and thus exerted antibody-targeting MCR cytotoxicity in vitro.</p> No potential conflict of interest relevant to this article was reported.