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ID 16768
Eprint ID
16768
フルテキストURL
Thumnail 94_359.pdf 507 KB
タイトル(別表記)
Studies on ferrous ion-induced lipid peroxidation Part Ⅱ Analysis of the induction period (lag) of ferrous ion-induced lipid peroxidation
著者
橋本 啓二 岡山大学医学部放射線医学教室
抄録
The author measured simultaneously Fe(Ⅱ)-induced peroxidation of lipid extracted from rat liver mitochondria by the TBA method and the concentration of Fe(Ⅱ) added to the medium quantitatively using Nitoroso-PSAP reagent. 1) An induction period (lag) at the early phase of the reaction of Fe(Ⅱ)-induced lipid peroxidation was observed as well as in mitochondria. 2) After most of the Fe(Ⅱ) concentration had decreased, the TBA-value, after lagtime, increased sharply. 3) Phosphate added to the medium shortened the lag-time. The lag-time in the presence of phosphate varied with the concentration and pH. However, in each case, there was a correlation between the decrement of Fe(Ⅱ) concentration and the length of lag time as described above. 4) By adding EDTA for various reaction times, Fe was chelated immediately, then the TBA value did not increase. 5) The results suggest: iron interacted with the chain reaction of lipid peroxidation, mainly as Fe(Ⅱ) and/or partially Fe(Ⅲ); Fe(Ⅱ) maintains the lag as a inhibitory action to the resolution into malondialdehyde(MDA) from lipid peroxide; Fe(Ⅲ) was required for the formation of MDA. 6) Using these results, the author discussed the mechanisms of Fe(Ⅱ)-induced lipid peroxidation.
キーワード
ミトコンドリア脂質
脂質過酸化反応
発行日
1982-04-30
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
94巻
3-4号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
359
終了ページ
368
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/94/3-4/94_3-4_359/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/index.html
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma