JaLCDOI 10.18926/AMO/31948
フルテキストURL fulltext.pdf
著者 Bralic, Marina| Muhvic-Urek, Miranda| Stemberga, Valter| Golemac, Mijo| Jurkovic, Slaven| Borcic, Josipa| Braut, Alen| Tomac, Jelena|
抄録 <p>The effects of irradiation on different cell compartments in the submandibular gland were analyzed in adult C57BL/6 mice exposed to X-ray irradiation and followed up for 10 days. Apoptosis was quantified using the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-digoxigenin nick end labeling method (TUNEL). Cell proliferation was detected using immunohistochemistry for proliferating cell nuclear antigen (PCNA). Radiation-induced apoptosis occurred rapidly, reaching a maximum 3 days post-irradiation. The percentage of apoptotic cells increased with the irradiation dose. At day 1 post-irradiation, cell proliferation was significantly reduced in comparison to sham-irradiated controls. After post-irradiation arrest of the cell cycle, proliferation increased in all gland compartments, reaching a maximum at day 6 post-irradiation. The proliferation response corresponded to the dose of irradiation. We suggest that the reason for gland dysfunction could be the coexistence of high apoptotic and proliferative activity in the irradiated gland.</p>
キーワード apoptosis early post-irradiation phase proliferation submandibular gland
Amo Type Article
発行日 2005-08
出版物タイトル Acta Medica Okayama
出版者 Okayama University Medical School
開始ページ 153
終了ページ 159
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 16155641
Web of Science KeyUT 000231478000006