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ID 31989
JaLCDOI
フルテキストURL
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著者
Ishii, Yasushi Okayama University
Shimomura, Hiroyuki Okayama University
Ito, Mamoru Okayama University
Miyake, Masanobu Okayama University
Ikeda, Fusao Okayama University
Miyake, Jiro Okayama University
Fujioka, Shin-ichi Okayama University
Iwasaki, Yoshiaki Okayama University ORCID Kaken ID
Tsuji, Hideyuki Okayama University
Tsuji, Takao Okayama University
抄録

It has been documented that the serum complement activities measured by hemolytic assay (CH50) are decreased after storage of sera at a low temperature in some patients with chronic hepatitis C. However, the mechanism of this phenomenon has not been identified yet. Here, we tried to elucidate factors involved in the cold activation of complement (CAC). To clarify what pathway is activated in CAC, we measured complement cleavage products after cold storage of sera. C4d increased significantly after 12 h-storage at cold temperatures in 5 CAC (+) sera compared with 5 CAC (-) (P < 0.01) and 3 control sera (P < 0.05), while Bb did not increase in any of the groups. In order to determine whether IgG or IgG complex is necessary for CAC, 8 CAC (+) sera were incubated with Protein G Sepharose gel beads, and all of them retained hemolytic activities to some extent after cold storage. Column chromatography through Superose 6HR of CAC-positive serum identified the fractions containing molecules that induced CAC in normal serum, which were depleted by treatment with protein G Sepharose. In conclusion, CAC in hepatitis C seems to occur via a classical or lectin pathway, and the IgG complex produced in hepatitis C virus infection may be an important factor in inducing CAC, a common extrahepatic manifestation of hepatitis C.

キーワード
hepatitis C virus
chronic hepatitis
complement activation
Amo Type
Article
発行日
2001-08
出版物タイトル
Acta Medica Okayama
55巻
4号
出版者
Okayama University Medical School
開始ページ
229
終了ページ
235
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT