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ID 31685
JaLCDOI
フルテキストURL
著者
Kondo, Junichi Okayama University
Shimomura, Hiroyuki Okayama University
Fujioka, Shin-ichi Okayama University
Iwasaki, Yoshiaki Okayama University
Takagi, Shinjiro Okayama University
Ohnishi, Yasuhiro Okayama University
Tsuji, Hideyuki Okayama University
Sakaguchi, Kosaku Okayama University
Yamamoto, Kazuhide Okayama University
Tsuji, Takao Okayama University
抄録

The preS2 region of the hepatitis B virus (HBV) has been reported to have human polymerized albumin receptor (PAR) activity, which correlates with viral replication. Here, we studied the genomic sequence of the preS region from rare patients lacking PAR activity, despite active viral replication. PAR and DNA polymerase activity was identified in 178 HBe antigen-positive HBV carriers, and a significant correlation between 2 markers was shown, except in 2 hepatitis patients lacking PAR activity. Nucleotide sequences of the preS region of HBV from both patients were examined by direct sequencing of PCR products. In one patient, a 45-base deletion was found to overlap half of the putative polymerized human albumin binding site in the preS2 region. In the other patient, a point mutation at the first nucleotide of the start codon of the preS2 region of HBV was found. There was no such genomic change in the 3 control HBV sequences. These results indicate that the preS2 region is necessary for binding of polymerized human albumin, and this is the first report of naturally existing mutant virus with no or low PAR activity.

キーワード
hepatitis B virus
preS region
polymerized albumin receptor
genetic mutation
genetic deletion
Amo Type
Article
発行日
2002-08
出版物タイトル
Acta Medica Okayama
56巻
4号
出版者
Okayama University Medical School
開始ページ
193
終了ページ
198
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT