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ID 56198
フルテキストURL
著者
Takeda, Tetsuya Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Kozai, Toshiya Department of Physics, College of Science and Engineering, Kanazawa University
Yang, Huiran Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Ishikuro, Daiki Department of Physics, College of Science and Engineering, Kanazawa University
Seyama, Kaho Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Kumagai, Yusuke Department of Physics, College of Science and Engineering, Kanazawa University
Abe, Tadashi Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Yamada, Hiroshi Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Uchihashi, Takayuki CREST, JST
Ando, Toshio CREST, JST
Takei, Kohji Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
抄録
Dynamin is a mechanochemical GTPase essential for membrane fission during clathrin-mediated endocytosis. Dynamin forms helical complexes at the neck of clathrin-coated pits and their structural changes coupled with GTP hydrolysis drive membrane fission. Dynamin and its binding protein amphiphysin cooperatively regulate membrane remodeling during the fission, but its precise mechanism remains elusive. In this study, we analyzed structural changes of dynamin-amphiphysin complexes during the membrane fission using electron microscopy (EM) and high-speed atomic force microscopy (HS-AFM). Interestingly, HS-AFM analyses show that the dynamin-amphiphysin helices are rearranged to form clusters upon GTP hydrolysis and membrane constriction occurs at protein-uncoated regions flanking the clusters. We also show a novel function of amphiphysin in size control of the clusters to enhance biogenesis of endocytic vesicles. Our approaches using combination of EM and HS-AFM clearly demonstrate new mechanistic insights into the dynamics of dynamin-amphiphysin complexes during membrane fission.
キーワード
EM
HS-AFM
amphiphysin
biophysics
cell biology
dynamin
human
in vitro reconstitution
membrane remodeling
structural biology
発行日
2018-01
出版物タイトル
eLife
7巻
出版者
eLife Sciences Publications
開始ページ
e30246
ISSN
2050-084X
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
論文のバージョン
publisher
PubMed ID
DOI
Web of Sience KeyUT
関連URL
isVersionOf https://doi.org/10.7554/eLife.30246