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ID 57388
フルテキストURL
著者
Yamanashi, Taro Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Maki, Misayo Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Kojima, Keiichi Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Shibukawa, Atsushi Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Tsukamoto, Takashi Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Chowdhury, Srikanta
Yamanaka, Akihiro
Takagi, Shin
Sudo, Yuki Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University ORCID 科研費研究者番号
抄録
Ion pumps and channels are responsible for a wide variety of biological functions. Ion pumps transport only one ion during each stimulus-dependent reaction cycle, whereas ion channels conduct a large number of ions during each cycle. Ion pumping rhodopsins such as archaerhodopsin-3 (Arch) are often utilized as light-dependent neural silencers in animals, but they require a high-density light illumination of around 1 mW/mm2. Recently, anion channelrhodopsins -1 and -2 (GtACR1 and GtACR2) were discovered as light-gated anion channels from the cryptophyte algae Guillardia theta. GtACRs are therefore expected to silence neural activity much more efficiently than Arch. In this study, we successfully expressed GtACRs in neurons of the nematode Caenorhabditis elegans (C. elegans) and quantitatively evaluated how potently GtACRs can silence neurons in freely moving C. elegans. The results showed that the light intensity required for GtACRs to cause locomotion paralysis was around 1 µW/mm2, which is three orders of magnitude smaller than the light intensity required for Arch. As attractive features, GtACRs are less harmfulness to worms and allow stable neural silencing effects under long-term illumination. Our findings thus demonstrate that GtACRs possess a hypersensitive neural silencing activity in C. elegans and are promising tools for long-term neural silencing.
発行日
2019-05-27
出版物タイトル
Scientific reports
9巻
1号
出版者
Nature Publishing Group
開始ページ
7863
ISSN
20452322
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
論文のバージョン
publisher
PubMed ID
DOI
関連URL
isVersionOf https://doi.org/10.1038/s41598-019-44308-x
ライセンス
http://creativecommons.org/licenses/by/4.0/