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ID 40754
フルテキストURL
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タイトル(別表記)
Molecular cloning and structural analyses of human DNA sequences related to the squirrel monkey retrovirus LTR
著者
長尾 一孝 岡山大学医学部癌源研究施設生化学部門
抄録
Sequences related to the squirrel monkey retrovirus (SMRV) LTR were isolated from a genomic library of human DNA by screening under conditions of relaxed stringency. The probe contains several transcriptional signals and its regulatory sequences in SMRV-H LTR, such as the enhancer sequence, CAT box, TATA box, and polyadenylation signal. More than 50 positive signals were detected in 4.4×105 recombinant phages. The cloned human sequences named SRH strongly hybridized with SMRV-H LTR and some of them weakly hybridized with SMRV-H structural genes. In SRH-1, the region related to SMRV-H prt-pol-env was flanked by the two separately located sequences related to the LTR. SRH-3 has a solitary LTR-related region, whereas, in SRH-5, the LTR-related region was adjacent to the pol-env-related sequence. The reliability of these hybridization experiments was confirmed by reciprocal hybridization. The restriction enzyme cleavage maps of these clones were different from those of known human endogenous retroviruses. Alu sequences, a human highly repetitive sequences, were inserted to the LTR-and pol-env-related regions in SRH-5.
キーワード
ヒト内在性レトロウイルス
リスザルレトロウイルス
Long terminal repeat
分子クローニング
発行日
1989-06
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
101巻
5-6号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
557
終了ページ
571
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/101/5-6/101_5-6_557/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma