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ID 13759
Eprint ID
13759
フルテキストURL
Thumnail 106_717.pdf 1.83 MB
タイトル(別表記)
Cloning and structural analysis of mouse Apex gene encoding the major apurinic/apyrimidinic endonuclease
著者
長尾 一孝 岡山大学医学部附属分子細胞医学研究施設病態分子生物学部門
抄録
The APEX nuclease is a mammalian multifunctional repair enzyme having 5' apurinic/apyrimidinic (AP) endonuclease, DNA 3' repair diesterase, 3'-5' exonuclease and DNA 3' phosphatase activities. The mouse Apex gene for the enzyme, was isolated from a mouse leukocyte genomic library by plaque hybridization with the mouse Apex cDNA as a probe. The nucleotide sequence of the Apex gene and its 5'-and 3'-flanking regions were determined. With reference to the published Apex cDNA sequence, the mouse Apex gene can be divided into five exons and four introns with a total length of about 2.6 kb. The boundaries between exon and intron follow the GT/AG rule. The translation initiation and termination sites are located in exons Ⅱ and Ⅴ, respectively. A part of the 5' flanking region belongs to a CpG island, which extends to intron Ⅱ. The CpG island is thought to be a putative transcription regulatory region of the Apex gene, a housekeeping gene.
キーワード
APEX ヌクレアーゼ
AP エンドヌクレアーゼ
DNA 修復酵素
Apex 遺伝子(マウス)
CpG island
備考
原著
発行日
1994-08
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
106巻
7-8号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
717
終了ページ
730
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/106/7-8/106_7-8_717/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/index.html
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma