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ID 40745
フルテキストURL
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タイトル(別表記)
Immunoelectron microscopic studies of human immunodeficiency virus antigens
著者
遠藤 悟 岡山大学医学部癌源研究施設生化学部門
抄録
Immunoelectron microscopic observations of human immunodeficiency virus (HIV) antigens were made using the indirect peroxidase-labeled antibody method with monoclonal antibodies (MoAb) to the envelope glycoprotein (gp160/120), matrix protein (MA, p17), and capsid protein (CA, p24). Antigens in the virions and HIV-infected cells were detected in frozen-sectioned specimens. Positive immunoreaction of MoAb to gp160/120 was located on the viral envelope, plasma membrane, endoplasmic reticulum, and nuclear membrane. The reaction of MoAb to p17 was at the inner leaflet of the viral envelope and the nucleoid. The reaction of MoAb to p24 was localized on and around the viral core. In cell suspension, however, positive immunostaining was observed only on the surface of the viral envelope and plasma membrane with MoAb to gp160/120, and no positive staining was observed with MoAb to p17 and MoAb to p24. The reaction of HIV-infected cells with normal control serum and all of the reactions in uninfected cells with these MoAbs were negative. It appears that antibodies have difficulty penetrating into virions and cells in cell suspension.
キーワード
ヒト免疫不全ウイルス
HIV抗原
構造蛋白質
免疫電顕
酵素抗体法
発行日
1989-06
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
101巻
5-6号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
459
終了ページ
471
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/101/5-6/101_5-6_459/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma