岡山医学会 Acta Medica Okayama 0030-1558 71 3-2 1959 Roller tube法による骨髄組織培養の吟味 第3編 血清及び腹水による影響について 附.全編の総括 1385 1403 EN Takashi Sugano After determining the optimal concentration of serum, the transudate and exudate of ascites in the abdominal cavity as the culture media in the rabbit bone-marrow tissue culture by the roller-tube method, the auther compared the effects of these three media on tissue culture: 1. As for serum the concentration at 20 per cent shows the best relative tissue growth rate and the highest wandering velocity of pseudoeosinophils and the least regressive degeneration, and therefore, this at 20 per cent concentration seems to be most suitable as the culture medium. Moreover, when the effect of the medium containing this 20% serum is compared with that of the control (the culture medium without serum), the former excels the latter in the effectiveness in every respects such as the relative tissue growth rate, the wandering velocity of pseudoeosinophils and the appearance of regressively degenerated granules. 2. As for the medium containing the transudate and exudate of the abdominal cavity, the rlative tissue growth rate in both cases is in an inverse proportion to the concentration, but the appearance of regressively degenerated granules is least at the concentration of 30 per cent, and cells are preserved in a good condition, proving that 30 per cent of the transudate or exudate of the abdominal cavity contained in the culture medium is most effective. Furthermore, in the comparison of the medium containing 30 per cent of either one of the two no great difference can be recognized, but the medium containing exudate is somewhat superior. When the effects of the medium containing transudate or exudate are compared with that of the control (the medium without either the transudate or exudate), the former clearly excels the latter in every respects such as in the relative tissue growth rate, the wandering velocity of pseudoeosinophils and the appearance of regressively degenerated granules. 3. On comparing the case of the medium containing serum with that containing the transudate or exudate of the abdominal cavity, the great difference can not be observed in the wandering velocity of pseudoeosinophils in all three cases, but in the case of the medium containing serum the relative tissue growth rate is greatest and also the appearance of regressively degenerated granules is least of all. Therefore, the former is fairly superior to either the medium containing the transudate or the medium containing exudate. 4. From these findings it seems that the medium containing the 20% serum, 15% chick-embryo extracts and 65% Gey's solution seems to be most suitable for the tissue culture by the roller-tube method. However, it is believed that the medium containing the 30% ascites, the 15% chick-embryo extracts and 55% Gey's solution can also be used to take the place of the above mentioned medium. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 71 3-2 1959 Roller tube法による骨髄組織培養の吟味 第2編 鶏胎児圧搾液による影響について 1373 1384 EN Takashi Sugano In the rabbit bone-marrow tissue culture by the roller-tube method, the author determined the optimal concentration in the medium of both the chick-embryo extracts and the first diluted solution from the sediment of the chick-embryo from which extracts was taken, and compared the effects of these two solutions on tissue culture. 1. As for the chick-embryo extracts, the relative tissue growth rate increases in a direct proportion to the concentration of the extracts in the medium, and at the concentration of 15 per cent, the wandering velocity of pseudoeosinophils is better, and the duration of wandering capacity is longest as well as the regressive degeneration of cells is least ; and therefore, the chick-embryo extracts at the concentration of 15 per cent is the optimal concentration for the medium of this bone-marrow tissue culture. 2. The first diluted solution from the sediment of the chick-embryo from which extracts taken likewise affects the tissue culture almost in the same way as the chick-embryo extracts; namely, the relative tissue growth rate increases in a direct proportion to the concentration, and at the concentration of 20 per cent the wandering velocity of pseudoeosinophils is better and also the regressive degeneration is least. Therefore, the optimal concentration in the medium of the first diluted solution from the sediment is at 20 per cent. 3. As for the comparative effects of the 15% chick-embryo extracts against the 20% diluted solution from the sediment, the former surpasses the latter in every respects, namely, in the relative tissue growth rate, the wandering velocity of pseudoeosinophils and the lowness in the appearance of regressively degenerated granules. Consequently it is thought that the 15% chick-embryo extracts is the most suitable culture medium for the rabbit bone-marrow tissue culture by the roller-tube method. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 71 3-2 1959 Roller tube法による骨髄組織培養の吟味 第1編 各種塩類溶液の影響について 1359 1372 EN Takashi Sugano By performing a series of rabbit bone-marrow tissue culture, the author compared with various plasmas, the fixed position of thin rectangular coverglass which was introduced into the test tube, and various salt solutions; and obtained the following results. 1. When rabbit plasma is used as the plasma clot for this method of tissue culture, the tissue growth rate is greater than that in the case of chicken plasma, but it is apt to cause liquefaction and fusion of the plasma clot and also makes it difficult to observe the cell structure. In contrast to this, with the use of chicken plasma, although the tissue growth rate is lower than in the case of the former, the regressive degeneration of cells is less than the former and the cell structure is maintained distinctly. Therefore, the latter plasma is more suitable for such a tissue culture. 2. As for the position of fixing the thin rectangular coverglass, it is best to fix it in such a way so that the explant placed on the coverglass is at the maximum distance from the inner wall of the testtube, when it is fixed at the minimum distance from the inner wall of the testtube, it gives the worst results; while when the coverglass is not fixed, it gives intermediate results. 3. As for the solutions to be used in the medium, Gey's solution is excellent from the standpoints of the rate of tissue growth, the wandering velocity of pseudoeosinophils and the rate of appearance of regressively degenerated granules for the effectiveness in tissue culture, followed by Hanks's solution, Tyrode's solution, Ringer's solution and physiological saline solution, in the order mentioned. However, no marked differences can be observed among the first three solution. No potential conflict of interest relevant to this article was reported.