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ID 55190
Sort Key
11
タイトル(別表記)
Identification of Marker Gene of Pars Tuberalis Morphogenesis in Chicken Embryo. The expression of Cytokine-like 1 and Gap junction protein alpha 5 in the pars tuberalis
フルテキストURL
著者
相澤 清香 岡山大学大学院自然科学研究科
檜垣 佑理子 岡山大学大学院自然科学研究科
御輿 真穂 岡山大学大学院自然科学研究科
竹内 栄 岡山大学大学院自然科学研究科高次生物科学講座・生体統御学分野
高橋 純夫 岡山大学大学院自然科学研究科高次生物科学講座・生体統御学分野
抄録
Adenohypophysis delivered from oral ectoderm consists of pars distalis (PD), pars intermedia and pars tuberalis (PT). The mechanisms of development of PD has been well studied, and the cell differentiation of PD has been well understood. However, the morphogenesis and the differentiation of PT are still unclear, and the gene expression during the PT development remains largely unknown. In this study, we explored the specifically expressing genes in PT during development and analyzed its spatiotemporal expressions pattern. Microarray analysis on laser-captured PT and PD tissues obtained from chicken embryos on embryonic day 10 (E10.0) showed high expressing genes, Cytokine-like 1 (CYTL1) and Gap junction protein alpha 5 (GJA5) in PT. A detail analysis of spatiotemporal expressions pattern during chick embryo development by in situ hybridization revealed that CYTL1 mRNA was first detected in lateral head ectoderm and ventral head ectoderm in E1.5. The CYTL1 expressions moved into Rathke’s pouch at E2.5, then it was localized in PT primordium and continuously expressed in PT primordium until E12.0. On the other hand, GJA5 mRNA was transiently detected in PT primordium from E6 to E14.0, while the expression was not detected in PD during development. These results suggested that these genes may be involved in the regulation mechanism of PT development and could be a useful marker in the PT development.
備考
寄稿(Contribution)
出版物タイトル
岡山実験動物研究会報
発行日
2017-04
33巻
出版者
岡山実験動物研究会
出版者(別表記)
Okayama Association for Laboratory Animal Science
開始ページ
31
終了ページ
34
資料タイプ
学術雑誌論文
OAI-PMH Set
岡山大学
言語
Japanese
論文のバージョン
publisher
査読
無し
Eprints Journal Name
poalas